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Vol 19, No 5 (2016)

Articles
Molecular genotypic detection of beta human papillomavirus DNA in the diagnosis of their association with certain skin epithelial neoplasias in immunocompromised and immunocompetent patients in the mode Real-Time
Korneva L.V., Snarskaya E.S., Nodelman E.K.
Abstract
Epidemiological and molecular biological data suggest that the beta human papillomavirus (HPV) can cause the development of a number of epithelial skin tumors, but this interconnection is currently not fully understood. Besides detection of beta HPV, it is necessary to measure and quantify the viral load, it is a new approach in the diagnosis of HPV infection. 52 patients were examined to identify the association of epithelial neoplasias beta human papilloma virus in immunocompromised and immunocompetent patients in the department of chronic hemodialysis and kidney transplantation of Moscow Regional Research and Clinical Institute. HPV detection was performed by polymerase chain reaction (PCR) with hybridization-fluorescence detection in real time (Real-Time). Amplification and detection were performed on the tool Rotor-Gene 3000 (“Corbett Research”, Australia). For quantification of beta HPV recombinant plasmid positive controls, as well as control plasmidfragment offi-globin human gene (FSIS CRIE Rospotrebnadzor) were used. In fibroepithelial polyp and in apparently healthy skin of immunosuppressive patients - renal transplant recipients (RPT) with a high frequency was detected beta HPV DNA - 64 and 54%, respectively, compared to healthy skin (47% of cases). In fibroepithelial polyp in 57% of immunosuppressive RPT mixed infection was revealed. Viral load in the fibroepithelial polyp was higher than that as in apparently normal skin and in donors skin. The high detection ofHPVDNA in RTP, as in the fibroepithelial polyp and in apparently healthy skin was found. Immunocompetent patients had high HPV DNA detection only in fibroepithelial polyp compared with apparently healthy skin.
Russian Journal of Skin and Venereal Diseases. 2016;19(5):260-265
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Dependence of the microRNA profile in cutaneous melanoma on clinical and morphological characteristics of the tumor
Komina A.V., Palkina N.V., Aksenenko M.B., Ruksha T.G.
Abstract
Melanoma is an aggressive skin cancer that is characterized by rapid growth and high invasion. Today extensive studies of melanomagenesis allow to subdivide this tumor into subtypes in accordance to molecular, genetic and biochemical characteristics in addition to standard histological classification for developing personalized approaches of treatment. One ofsuch molecular peculiarities is the expression profile of microRNAs in tumor cells alterations of which were shown during tumor progression. The aim of this study was to identify the correlation between the levels ofmicroRNA in melanoma cells, and the clinical and morphological characteristics of the disease. The comparative study of microRNAs expression profiles in melanoma is made by gender, age ofpatients, tumor Breslow’s thickness and histological subtypes. It was shown that the gender and age ofpatients have no effect on the expression of microRNA in melanoma. No correlation between the microRNA levels and tumor Breslow’s thickness was revealed. But it was observed a significant alteration between the microRNA profiles in the different histological melanoma subtypes. The significant change in the profile of microRNAs for superficial-spreading melanomas compared to other subtypes was shown that was associated with the processes of tumors development, as well as ErbB-, MAPK-, TGF-β-signaling pathways. Further study of the molecularfeatures of the superficial-spreading melanoma in combination with other characteristics might be a new step towards personalized medicine.
Russian Journal of Skin and Venereal Diseases. 2016;19(5):266-272
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Acne, the microbiome and innate immunity
Ionescu M., Feuiolley M., Enault J., Wolkenstein P., Robert G., Lefeuvre L.
Abstract
In the laste years several articles focalized on human microbioma - the microorganisms from skin, mucosae, bowel - and on its role in chronic inflammatory diseases of the skin as acne, rosacea, atopic dermatitis, seborrheic dermatitis. In this article the authors present an update on particular acne skin’s microbioma, on innate immunity in acne and new physiopathology mecanisms described in inflammatory process in acne, and at the end we present in vitro, ex vivo and in vivo studies on the microbioma modulation and microbiofilm of pathogenic ribotypes of P. acnes leading to a significant improvement of acne in a series of 74 acne patients.
Russian Journal of Skin and Venereal Diseases. 2016;19(5):272-278
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Acne, the microbiome and innate immunity
Ionescu M.A., Feuiolley M., Enault J., Wolkenstein P., Robert G., Lefeuvre L.
Abstract
In the laste years several articles focalized on human microbioma - the microorganisms from skin, mucosae, bowel - and on its role in chronic inflammatory diseases of the skin as acne, rosacea, atopic dermatitis, seborrheic dermatitis. In this article the authors present an update on particular acne skin’s microbioma, on innate immunity in acne and new physiopathology mecanisms described in inflammatory process in acne, and at the end we present in vitro, ex vivo and in vivo studies on the microbioma modulation and microbiofilm of pathogenic ribotypes of P. acnes leading to a significant improvement of acne in a series of 74 acne patients.
Russian Journal of Skin and Venereal Diseases. 2016;19(5):279-282
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Expression of protein p27 in various forms of seborrheic keratosis
Aleksandrova A.K., Sukolin G.I., Smolynnikova V.A.
Abstract
The significant role in the pathogenesis of seborrheic keratosis (SK) plays the violation of the cell cycle regulation. According to the research unit the cell proliferation in acanthotic and irritated histological types of SK is regulated by p27 (Kip1), cyclin-dependent kinase inhibitor. Considering the variety of histological types of SK, definition of p27 expression will reveal the characteristic features of the cell cycle disorders and proliferation for each type of tumor. Material and Methods. Of the 102 tumors from patients with SK, according to the results of histological examination, were selected 10 specimens of each histological types of SK: acanthotic, hyperkeratotic, adenoid, irritated, and clonal. We assessed all specimens for p27 (Kip1) expression using immunohistochemistry (monoclonal antibody p27 at a dilution of 1:20 (Novocastra Laboratories Ltd.). Three skin biopsy samples of healthy individuals were included. Results. Severe diffuse nuclear expression of p27 was present in all cases of adenoid, irritated, and 4 cases of clonal histological types of SK. In other tumor types positive reaction with monoclonal antibody to p27 was reduced as compared with healthy skin were recorded single positively stained nuclei of basal cells. Conclusions. Thus, we have found a violation of p27 protein expression in all types of seborrheic keratosis as with the excess (adenoid, irritated, clonal SK type) and in p27 protein deficiency (acanthotic, hyperkeratotic) normal course of cell cycle phases is broken. This leads to the disappearance of control over the cell proliferative activity and apoptosis, facilitating a slow, uncontrolled growth of SK cells.
Russian Journal of Skin and Venereal Diseases. 2016;19(5):283-286
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Clinical and pathological red flat depriving association
Molochkova Y.V., Khlebnikova A.N.
Abstract
Knowledge of the morphological structure of atypicalforms of lichen planus is necessaryfor the correct diagnosis of lichen planus. Due to the large number of atypicalforms, diagnosis often requires a histological examination of the affected skin. The article describes pathological features of typical and some atypical forms of lichen planus, observed in recent years in the department of dermatology of Moscow Regional Research and Clinical Institute.
Russian Journal of Skin and Venereal Diseases. 2016;19(5):286-290
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Diagnostic features of chronic urticaria
Kochergin N.G., Kolkhir P.V., Kosoukhova O.A.
Abstract
Urticaria is etiologically heterogeneous disease, where the main clinical sign is a wheal. For the duration of the disease course secrete acute and chronic urticaria. If urticaria elements in the skin of the patient are observedfrom several hours to 6 weeks, it is classified as acute urticaria, cutaneous if the process continues for more than 6 weeks, it is a chronic urticaria. The aim of our work was the optimization of diagnosis of chronic urticaria on the basis of the study of clinical and allergological and immunological characteristics of the testing results. The study involved 60 patients between the ages of 18 to 73 years with confirmed clinical diagnosis of chronic urticaria. The study was conducted in the clinic of skin and venereal diseases ofI.M. Sechenov First Medical State Medical University. Conducted clinical and anamnestic examination: medical history, assessment of complaints, determining the severity of urticaria (UAS7), quality of life (CU-Q2oL, DLQI), control of the symptoms of urticaria (UCT); laboratory tests: clinical blood test, C-reactive protein, thyroid hormones and antibodies to thyroid structures, tests to exclude physical urticaria, autologous serum skin test, D-dimer, rheumatoid factor, eosinophil-cationic protein, the total of IgE, antinuclear antibodies , C3 / C4 complement components, protein fractions, coagulation, urinalysis, general analysis offeces, feces on eggs of worms and protozoa. The study was conducted within 1 month and included diagnostic consultation period and 5 consultations every 7 days. Identified specific clinical and laboratory features that can be assigned to the diagnostic criteria for autoimmune form of chronic urticaria, which will continue to pick up these patients rational treatment. The high prevalence of urticaria, a variety offorms of the disease, the presence ofpathology predominantly in patients of working age, often ineffective diagnostic actions cause the urgency of the problem and the need for further study of this disease.
Russian Journal of Skin and Venereal Diseases. 2016;19(5):290-295
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Study of the effect of menopausal hormone therapy in women with psoriasis type 2 in postmenopause
Opruzhenkova E.P., Sidorenko O.A., Anisimova L.A., Starostenko V.V.
Abstract
The results of comparative analysis of the hormonal status from 75 women aged from 49 to 60 years old (average age 54.04 ± 1.28 year) in postmenopause with psoriasis type 2 (study group) and 50 women of control group of the same age and menstrual status without psoriasis are presented. There were two subgroups in the main group: subgroup 1 received basic therapy in combination with menopausal hormone therapy (MHT) (estradiol 1 mg + drospirenone 2 mg), subgroup 2 - basic therapy. Psoriatic process was assessed according to PASI index and was evaluated by the dermatology quality of the life index (DIQL) and the content of gonadotropic and sex hormones before treatment and after 3 and 6 months of starting treatment in patients of both subgroups. More pronounced clinical efficacy in combination with reduced FSH level is 13.2% (p < 0.001), increased content of estradiol 69.3% (p < 0.001) was observed in patients from 1 subgroup. MHT supplement to the basic treatment decreased the PASI index after 3 months of starting treatment for 50.9% (p fter 6 months - at 94% (p < 0.001), and reduced DIQL in subgroup 1 compared to 2 subgroup after 3 months - 15.3% (p < 0.05) and after 6 months - 20.8% (p < 0.05). In postmenopausal women with psoriasis favorable changes of indexes reflecting the clinical manifestations of the disease were associated with the correction of hormonal status in MHT estradiol 1 mg + drospirenone 2 mg.
Russian Journal of Skin and Venereal Diseases. 2016;19(5):296-300
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The role of correction of metabolic disorders in the treatment of androgenetic alopecia in women
Perlamutrov Y.N., Antsiferov M.B., Levina N.V.
Abstract
The problem of androgenetic alopecia in women is considered by experts of various specialties, however there are few reviews about metabolic processes in these patients. Objective: to increase the efficiency of treatment of androgenetic alopecia in view of metabolic abnormalities. Material and methods. 80 female patients with androgenetic alopecia were examined to identify the features of the clinical course and hormonal metabolic deviations. Results. It was found that women with the androgenetic alopecia have abnormalities in carbohydrate and lipid metabolism in the majority of cases. Combined therapy with metformin helped to increase the clinical efficacy in the treatment of women with androgenetic alopecia.
Russian Journal of Skin and Venereal Diseases. 2016;19(5):300-304
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Cromhidrosis: clinical observation
Tarasenko G.N., Kuzmina Y.V., Sysoeva A.N.
Abstract
The clinical case of apocrine sweat glands disease - chromhidrosis, that is characterized by stains on linen and clothes in armpit, brushes, genitals, is presented. Stains can be various color, it depends on the reason that causes the pathology. Authors pay attention of doctors to necessity of inspection of such patients taking into account the anamnesis and a possible pathology of internal organs.
Russian Journal of Skin and Venereal Diseases. 2016;19(5):305-307
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Chronic spontaneous urticaria
Minkina E.V.
Abstract
Urticaria is a common disorder. Urticaria ranks 3rd place in Russia after bronchial asthma and pollinosis. The incidence of the disease is 15.3-31%, with 41.3% of the chronic urticarial and 58.7% of the angioedema. The detection of cause of the disease and appropriate treatment is still a matter of some difficulties. The cause of the chronic spontaneous urticaria is not identified in 80-85%. The literature review of data on the main aspects of etiology, nosogenesis, diagnostic, international and local recommendations for the treatment of chronic spontaneous urticarial is presented.
Russian Journal of Skin and Venereal Diseases. 2016;19(5):307-310
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Biorevitalizant with multitargeted action
Lipova E.V., Suhovei Y.G., Griazeva N.V.
Abstract
Biorevitalization is an intradermal injection of unstabilized hyaluronic acid to improve skin texture. The article presents the results of the drug Revi for the treatment of rosacea and biological aging by stimulating its internal physiological reserves and it can be recommended in conditions such as rosacea, the biological aging of the skin.
Russian Journal of Skin and Venereal Diseases. 2016;19(5):311-317
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The opening of the Russian-Israeli scientific and educational center “Laser Dermatology and Cosmetology”
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Russian Journal of Skin and Venereal Diseases. 2016;19(5):318-320
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