Vol 22, No 5-6 (2019)

In recent decades, interest in the role of human papillomavirus (HPV) has been steadily increasing, which can be attributed both to the evolution of molecular genetic detection methods and to the widespread of this viral infection in the population. Epidemiological and molecular biological data suggest that HPV genus beta can cause the development of a number of epithelial non-melanocytic neoplasms of the skin. However, this relationship has not yet been fully studied. Possibly, human papillomavirus infection should be considered from the perspective of co-carcinogenesis with the cumulative effect of UV irradiation, which is indirectly indicated by the predominant localization of elements in open areas of the skin and the high risks of their malignant transformation.

Psoriatic onychodystrophy is a comorbidity that often occurs in patients with psoriasis, affecting their quality of life. Although success has recently been achieved in the treatment of the cutaneous form of psoriasis, there are few publications about nail therapy with proven effectiveness. The development of effective and safe therapies remains relevant for nail psoriasis. Objective. to evaluate the effectiveness and tolerability of the fixed combination of betamethasone dipropionate 0.05%/calcipotriol monohydrate 0.005% gel and clobetasole propionate 0.05% ointment in patients with psoriatic onychodystrophy. Material and methods. the study included 50 patients with psoriasis vulgaris, mild to moderate severity with lesions of the nail plates. Patients were divided into two groups and used betamethasone dipropionate 0.05%/calcipotriol monohydrate 0.005% gel (group A) and clobetasole propionate 0.05% ointment with occlusion (group B) for 24 weeks with hardware treatment of nail plates at 0, 8, 16 weeks. They did not receive any other local or systemic treatment. The severity of nail psoriasis was assessed by the NAPSI index at week 0, 8, 16, and 24, and by the IGA after treatment. results. 43 patients completed the study. At the beginning of the study, the average NAPSI was 32.8 ± 10.3 in group A and 33.1 ± 9.8 in group B. At week 24 the average NAPSI score decreased to 12.8 ± 7.2 in group A and 11.8 ± 68 in group B. IGA for evaluation treatment score was 3 ± 0.74 in group A and 3.1 ± 0.79 in group B. Conclusions. Combined treatments in the form of hardware processing nail plates with betamethasone dipropionate 0.05%/calcipotriol monohydrate 0.005% gel and clobetasol propionate 0.05% ointment are effective and can be used in patients with moderate and severe nail psoriasis, which is confirmed by the results in clinical improvement of 60.8% and 64.5%, respectively, based on a decrease in NAPSI at 24 weeks and an assessment of the therapeutic response after treatment using the IGA.

Antioxidant system dysfunction is a significant factor in the pathogenesis of inflammatory dermatosis. Despite an impressive number of studies on the causes and chronization of microbial eczema, the development of a comprehensive approach to therapy taking into account metabolic disorders remains a pressing task. Material and metods. In the course of this study, the effectiveness and expediency of including in microbial eczema therapy a preparation of antioxidant action of dimethyloxobutylphosphonyldimethylate (DFD) was evaluated based on the dynamics of antioxidant system dysfunction indices, markers of syndrome of endogenous intoxication and clinical manifestations. The material for the study was the blood of patients with microbial eczema taken before and after treatment. Dynamics of parameters of enzymatic and non-enzymatic units of antioxidant system dysfunction, level middle mass molecules were analyzed against the background of standard therapy and with inclusion of DFD. Results. Inhibition of antioxidant system dysfunction activity compared to the control group was detected in the blood of all microbial eczema patients prior to treatment. No antioxidant status was observed after the standard therapy course despite achieving clinical improvement. The inclusion of DFD in complex microbial eczema therapy had a positive effect on the state of antioxidant system dysfunction and the dynamics of the skin pathological process.

Objective. the determination of ibuprofen, paracetamol, nimesulide, amoxicillin, ceftriaxone effect on barium sulfate-stimulated luminol-dependent chemiluminescence of leukocytes (SLHL) in patients with drug toxidermy, which occurred after taking these drugs, and the control group for the selection of the drug for replacement therapy. Material and methods. there were 67 patients (44 women and 23 men) with drug toxidemia. 22.4% patients had drug toxidermia after taking ibuprofen, 20.9% patients had drug toxidermia after taking paracetamol, 19.4% patients had drug toxidermia after taking nimesulide, 20.9% patients had drug toxidermia after taking amoxicillin, 16.4% patients had drug toxidermia after taking ceftriaxone and a control group consisting of 67 people (42 women and 25 men), who previously took these drugs without any adverse reactions. The study participants were aged 18 to 75 years, median 53. Measurement SLHL of leukocytes was performed on the Lum-100 chemiluminometer. The calculations were carried out using the computer program «PowerGraph». Results. during the influence of ibuprofen, paracetamol, nimesulide, amoxicillin, ceftriaxone on the blood of patients with intolerance to these drugs and the control group dose-dependent changes in SLHL were observed. The relative intensity and light sum of blood SLHL in patients were significantly lower than in the control group at all studied drug concentrations (p < 0.01). The relative intensity and light sum of blood SLHL in patients were significantly lower in samples with the drug that caused the development of the disease than with drugs for replacement therapy (p < 0.01). At a minimum concentration of these drugs, there are no significant differences between these indicators (p > 0.05). Conclusion. During the influence of ibuprofen, paracetamol, nimesulide, amoxicillin, ceftriaxone on the blood of patients with intolerance to these drugs and the control group, a dose-dependent change in SLHL is observed. The relative intensity and light sum of SLHL blood in patients are significantly lower than that of the control group at all studied concentrations of the drug (p < 0.01). The relative intensity and light sum of blood SLHL in patients were significantly lower in samples with the drug, which caused the development of the disease, in the maximum concentration than with drugs for replacement therapy (p < 0.01). In the minimum concentration, there were no significant differences in indicators (p > 0.05). The revealed significant differences in SLHL indices allow applying the chemiluminescent method for the diagnosis of intolerance to ibuprofen, paracetamol, nimesulide, amoxicillin, ceftriaxone and for a personalized selection of drugs for replacement therapy.

The radio wave effect on biological tissues is of great interest to dermatologists, cosmetologists, and plastic surgeons. The modern information about radio wave therapy of involutional changes of face and neck skin is shown. A literature review of the physical aspects of the high-frequency current of the radio wave range is presented. The thermal and oscillation effects of radio frequency (RF) on biological tissues are shown, the mechanism of these effects at the cellular level is described. Primary targets of high-frequency currents exposure in the skin, their effect on the physicochemical properties of collagen and unicellular organisms are described. The mechanism of pulsed and continuous radio wave technologies and the possibility of their use in medical practice are described. Types of minimally invasive and non-invasive electrodes, when the choice of electrode diameter determines the therapy of specific clinical cases, are shown. The multifunctional minimally invasive RF device Innofill with needle and cannula active electrodes is presented. The technical characteristics and exposure modes of the device are considered. The features of the cannula electrode and the possibilities of its use in solving various dermatological and cosmetic problems are highlighted. A histological study of a combined technique of the Innofill radio wave method with the filler based on hyaluronic acid, was performed. A change in the amount of collagen and elastin fibers was noted. The distribution of the filler in the tissues was evaluated after the injection of the filler based on hyaluronic acid, after 30 and 60 seconds of RF exposure and after 30 seconds of RF exposure in a tunnel technique with the formation of a collagen channel. Histological material was stained with hematoxylin-eosin, Masson and Van Gieson trichrome. High efficiency, safety and prolonged effect of this exposure were described.