Development and application of a multicomponent film biocoating based on sodium carboxymethylcellulose to study the antibacterial activity of microflora in the oral fluid of patients with pemphigus vulgaris in vitro

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Abstract

BACKGROUND: A special place among dermatological diseases is occupied by dermatoses affecting the oral mucosa, in particular pemphigus vulgaris. Constant damage to the mucous membrane, combined with the presence of abundant microflora in the oral cavity, leads to rapid variability in the primary or pathognomonic manifestations of specific diseases, making them look similar. Erosion and ulcers in the oral cavity are very difficult to treat and are accompanied by severe pain. The role of the oral microflora in the development and progression of pemphigus vulgaris has not been fully studied, however, it is known that the microflora of patients differs significantly from that of healthy individuals. Today, much attention is paid to studying the composition, properties and role of the microflora of the oral mucosa in the manifestation and course of pemphigus vulgaris.

AIM: determination of the most effective combination of a three-component film biocoating based on sodium carboxymethylcellulose for patients with pemphigus vulgaris, by assessing and comparatively in vitro comparison of quantitative and qualitative characteristics of the oral microflora, as well as local oral protective factors (lysozyme titer, phagocytosis index neutrophils and the level of the secretory fraction of immunoglobulin class A)

MATERIALS AND METHODS: A cross-sectional single-center study was performed among patients with pemphigus vulgaris (n=12). Oral fluid was collected by washing (rinsing) 4.5 ml of physiological solution from the oral mucosa. Subsequently, in the laboratory, a series of serial dilutions were prepared, some of which were inoculated by quantitative sectoral sowing on media intended for the cultivation of aerobic and anaerobic microbes. Microbiological and immunological sensitivity to films with different concentrations of mometasone furoate (20–40–80 mg) and propolis (2.5–5–7.5–10%) was determined.

RESULTS: The most pronounced antibacterial activity was observed in the following 2-component forms (gel film and propolis at a concentration of 7.5%; gel film and mometasone furoate in an amount of 20 mg) and in 3-component gel films (gel film, propolis at a concentration of 5% and mometasone furoate 20 mg). According to the results of a comparative quantitative assessment of local factors protecting the oral cavity in patients with pemphigus vulgaris, their positive dynamics, characterized by the restoration of immunodeficiency, were most influenced by the three-component composition, including a gel film, propolis 5% and mometasone furoate 80 mg.

CONCLUSION: The three-component composition of the film biocoating based on carboxymethylcellulose significantly reduces the quantitative and improves the qualitative indicators of microorganisms in the oral cavity, thereby having a positive effect on the treatment process for patients with pemphigus vulgaris.

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About the authors

Evelina V. Koldarova

Fergana Medical Institute of Public Health

Author for correspondence.
Email: koldarova7@gmail.com
ORCID iD: 0000-0001-9450-4004
SPIN-code: 5792-6185
Uzbekistan, Fergana

Akmal A. Sidikov

Fergana Medical Institute of Public Health

Email: medik-85@bk.ru
ORCID iD: 0000-0002-0909-7588
SPIN-code: 3812-8400

MD, Dr. Sci. (Medicine), Professor

Uzbekistan, Fergana

Bakhrambek I. Mukhamedov

Tashkent State Dental Institute

Email: mukhamedov69@gmail.com
ORCID iD: 0000-0002-7230-5183
SPIN-code: 8341-0974

MD, Cand. Sci. (Medicine), Assistant Professor

Uzbekistan, Tashkent

Ilaman M. Mukhamedov

Tashkent State Dental Institute

Email: dr.ilaman@mail.ru
ORCID iD: 0009-0003-1001-5767
SPIN-code: 3013-4505

MD, Dr. Sci. (Medicine), Professor

Uzbekistan, Tashkent

Abdushukur A. Sarymsakov

Institute of polymer chemistry and physics, Academy of sciences of the Republic of Uzbekistan

Email: sarimsakov1948@gmail.com
ORCID iD: 0000-0002-1345-5288

Dr. Sci. (Engineering), Professor

Uzbekistan, Tashkent

Akobirkhon I. o’g’li Shukurov

Institute of polymer chemistry and physics, Academy of sciences of the Republic of Uzbekistan

Email: shukuroov@gmail.com
ORCID iD: 0000-0002-2889-0258
Uzbekistan, Tashkent

Denis V. Zaslavsky

Saint-Petersburg State Pediatric Medical University

Email: venerology@gmail.com
ORCID iD: 0000-0001-5936-6232
SPIN-code: 5832-9510

MD, Dr. Sci. (Medicine), Professor

Russian Federation, Saint Petersburg

Igor A. Gorlanov

Saint-Petersburg State Pediatric Medical University

Email: gorlanov53@mail.ru
ORCID iD: 0000-0001-9985-6965
SPIN-code: 1195-6225

MD, Dr. Sci. (Medicine), Professor

Russian Federation, Saint Petersburg

Elizaveta D. Zaslavskaya

Saint-Petersburg State Pediatric Medical University

Email: zaslavliza@gmail.com
ORCID iD: 0000-0002-7434-3634
SPIN-code: 1218-8166
Russian Federation, Saint Petersburg

Darya V. Kozlova

Saint-Petersburg State Pediatric Medical University

Email: dashauchenaya@yandex.ru
ORCID iD: 0000-0002-6942-2880
SPIN-code: 3783-8565
Russian Federation, Saint Petersburg

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Supplementary files

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1. JATS XML
2. Fig. 1. Growth of Candida albicans on HiCrome Candida Differential Agar/Base (a) and on medium Saburo (b).

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3. Fig. 2. Growth of methicillin-resistant Staphylococcus aureus on HiChrome MRSa Agar/Base (a) and on yolk-salt agar (b).

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4. Fig. 3. Growth of Escherichia coli on media HiChrome E. coli Agar (a) and on medium Endo (b).

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5. Fig. 4. Growth of Staphylococcus aureus on media HiChrome Aureus Agar/Base (a) and on yolk-salt agar (b).

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