Optimization of the molecular genetics for the diagnosis of early mycosis fungoides

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Abstract

BACKGROUND: Mycosis fungoides (MF) is the most common disease among the cutaneous T-cell lymphomas (85–90%). The accuracy of the diagnosis of MF, which is confirmed only by clinical, histological and immunohistochemical signs, is 50–75%.

AIMS: To investigate genetic marker STAT4 for early diagnosis of mycosis fungoides.

MATERIALS AND METHODS: A study involving 42 patients with MF and chronic benign dermatoses (CBD) was performed. The analysis of gene expression STAT4 was carried out by TaqMan Real time-PCR. The objects of the study were lesional skin samples of patients. A group with MF consisted of 29 patients, a group with CBD consisted of 13 patients, a control group included 10 healthy volunteers.

RESULTS: The study revealed that the level of STAT4 gene expression showed a significant (9 times) increase in the expression of gene STAT4 in patients with MF (168.2) compared with patients with CBD (18.5; p <0.001) and 561 times — with healthy volunteers (0.3; p <0.001).

CONCLUSION: For early diagnosis of MF the level of expression STAT4 is of great importance. Inclusion of STAT4 in the list of diagnostic algorithm increases the accuracy of differential diagnosis of MF and CBD from 59.1 to 92.2%.

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About the authors

Olga Yu. Olisova

I.M.Sechenov First Moscow State Medical University (Sechenov University)

Email: olisovaolga@mail.ru
ORCID iD: 0000-0003-2482-1754
SPIN-code: 2500-7989

Dr. Sci. (Med.), Professor

Russian Federation, 8-2, Trubetskaya street, Moscow, 119992

Ekaterina V. Grekova

I.M.Sechenov First Moscow State Medical University (Sechenov University)

Author for correspondence.
Email: grekova_kate@mail.ru
ORCID iD: 0000-0002-7968-9829
SPIN-code: 8028-5545
https://journals.eco-vector.com/1560-9588/user/registerUser#formErrors

Assistant, Professor

Russian Federation, 8-2, Trubetskaya street, Moscow, 119992

Ekaterina A. Alekseeva

I.M.Sechenov First Moscow State Medical University (Sechenov University); Research Centre for Medical Genetics

Email: ekater.alekseeva@gmail.com

Cand. Sci. (Biol.), Senior Research Associate

Russian Federation, 8-2, Trubetskaya street, Moscow, 119992; Moscow

Dmitry V. Zaletaev

I.M.Sechenov First Moscow State Medical University (Sechenov University), Research Centre for Medical Genetics

Email: zalnem@mail.ru
ORCID iD: 0000-0002-9323-2673
SPIN-code: 9836-2326

Dr. Sci. (Biol.), Professor

Russian Federation, 8-2, Trubetskaya street, Moscow, 119992; Moscow

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Supplementary files

Supplementary Files
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1. Fig. 1. Expression of the STAT4 gene in patients with fungal mycosis and chronic benign dermatoses compared with healthy donors.

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2. Fig. 2. Diagnostic algorithm of early stages of fungal mycosis

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