Comparative histological assessment of the effect of retinol palmitate preparations on aging skin

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Abstract

BACKGROUND: Most often, the natural form of vitamin A (retinol palmitate) is used to prevent skin aging. There are two topical preparations in the form of ointment on the Russian market that contain a high concentration of retinol palmitate, including in combination with cholecalciferol and tocopherol acetate. The specific effects of these drugs on the structure of aging skin have not yet been fully studied.

AIM: Comparative histological assessment of the effect of topical preparations in the form of ointment containing retinol palmitate on the epidermis and dermis of women aged 40–55 years.

MATERIALS AND METHODS: The clinical study included 18 women aged 40 to 55 years old. Group 1 included 6 women who used retinol palmitate ointment 2 times a day on the skin of the face. Group 2 consisted of 6 women who used the dermatoprotective agent 2 times a day on the skin of the face which, in addition to retinol palmitate, includes cholecalciferol and α-tocopherol acetate. Group 3 consisted of 6 women and was an intact comparison group. The observation period is 90 days. Patients underwent a punch biopsy with histological examination of the skin before and after the observation period.

RESULTS: According to the results of the study, it was revealed that ointments with a high concentration of retinol palmitate have a stimulating effect on the proliferation of keratinocytes of the epidermis, which manifested itself in an in increasing the number of rows of cells of the prickly layer. In addition, under the action of these drugs, the synthetic activity of keratinocytes of the granular layer increases, which contributes to the proper formation of the stratum corneum of the epidermis. The use of ointments leads to a significant increase in the thickness of both the granular and prickly layers of the epidermis and the dermis.

CONCLUSION: Ointments with a high concentration of retinol palmitate improve the structure of aging skin.

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About the authors

Margarita G. Kostyaeva

Peoples' Friendship University of Russia

Email: kostyaeva.71@mail.ru
ORCID iD: 0000-0001-5182-0373
SPIN-code: 4364-3214

Cand. Sci. (Veterinary)

Russian Federation, Moscow

Olga А. Dolnikova

Chelyabinsk Regional Clinical Skin and Venereological Dispensary; South-Ural State Medical University

Email: dolnmoa74@mail.ru
ORCID iD: 0000-0002-2180-9170
SPIN-code: 5117-1840
Russian Federation, Chelyabinsk; Chelyabinsk

Stanislava Y. Petrova

Joint-stock company Pharmaceutical enterprise "Retinoids"; I. Mechnikov Research Institute of Vaccines and Sera

Author for correspondence.
Email: petrovastanislava@yandex.ru
ORCID iD: 0000-0003-3034-0148
SPIN-code: 7268-6944

MD, Cand. Sci. (Medicine)

Russian Federation, Moscow; Moscow

Konstantin V. Nozdrin

Joint-stock company Pharmaceutical enterprise "Retinoids"

Email: kvn@retinoids.ru
ORCID iD: 0000-0002-2943-3585
SPIN-code: 9884-3751

Cand. Sci. (Pharmacy)

Russian Federation, Moscow

Oleg R. Ziganshin

Chelyabinsk Regional Clinical Skin and Venereological Dispensary; South-Ural State Medical University

Email: ziganshin_oleg@mail.ru
ORCID iD: 0000-0002-5857-0319
SPIN-code: 5339-2533

MD, Dr. Sci. (Medicine), Professor

Russian Federation, Chelyabinsk; Chelyabinsk

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2. Рисунок 1. (Исправленная версия) Гистологические препараты (окраска гематоксилином и эозином)

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3. Fig. 1. Histological preparations (stained with hematoxylin and eosin): 1 ― the preparation No. 7, magnification 100; 2 ― the preparation No. 7, magnification 200: you can see the tight fit of the scales of the stratum corneum (black arrows); 3 ― the preparation No. 7, magnification 400: it can be seen that the keratinocytes of the granular layer contain abundant keratogyaline granules (black arrows); 4 ― the preparation No. 10, magnification 200: you can see the folded surface of the skin (white arrows); 5 ― the preparation No. 10, magnification 200: the increase in the rows of keratinocytes is clearly visible on the preparation (black arrow), the relief of the skin surface is more even (white arrow); 6 ― the preparation No. 14, magnification 800: the epidermis contains up to 15 rows of keratinocytes (black arrow), the granular layer is well defined (white arrows). РП ― retinol palmitate.

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