The relationship of microbial biodiversity and clinical forms of oral lichen planus: Analysis based on 16S rRNA sequencing
- Authors: Teplyuk N.P.1, Stepanov M.A.1, Damdinova B.S.1, Toshchakov S.V.2, Noskov S.A.3
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Affiliations:
- I.M. Sechenov First Moscow State Medical University (Sechenov University)
- National Research Centre "Kurchatov Institute"
- Национальный исследовательский центр «Курчатовский институт»
- Section: DERMATOLOGY
- Submitted: 18.03.2024
- Accepted: 19.05.2024
- Published: 19.05.2024
- URL: https://rjsvd.com/1560-9588/article/view/629200
- DOI: https://doi.org/10.17816/dv629200
- ID: 629200
Cite item
Abstract
BACKGROUND: The composition and changes of microbiota have a significant impact on overall health and the development of various diseases. Of particular relevance is the problem of changes in the oral microbiota in patients with lichen planus of the oral mucosa. Studying the relationship between the composition of the oral microbiota and the pathogenesis of oral lichen planus will improve the understanding of the mechanisms of this disease. Thus, this topic is of considerable interest to a wide range of specialists in the field of medicine and biology.
AIMS: detailed analysis of oral cavity microbiota and establishment of potential pathogenetic microbial associations with oral lichen planus.
MATERIALS AND METHODS: The study included samples from patients diagnosed with various forms of КПЛ СОПР(lichen planus erosive-ulcerative) and a control group. The investigation was based on analyzing microbial diversity metrics (alpha and beta diversity), relative abundance of bacterial taxa, and identification of unique bacterial taxa in the КПЛ СОПР patients. This analysis utilized the 16S rRNA sequencing method.
RESULTS: The analysis revealed a rich bacterial composition in patients with OLP, which was significantly different from that in the control group. Differences were also observed between the subgroups, especially between the typical and erosive-ulcerative forms of the disease. Notably, beta diversity did not show significant differences between the groups, indicating a similar overall microbiota composition despite fluctuations in the relative abundance of species. Nevertheless, the typical clinical form of the disease demonstrated more significant differences in the microbiota structure compared to the hyperkeratotic and erosive-ulcerative forms.
Furthermore, analysis of the study groups revealed the presence of 50% shared microbial species, while the other half was represented by unique species associated with OLP. Regarding the subgroups, it was found that unique microorganisms correlated with the typical and erosive-ulcerative forms, respectively, providing a deeper understanding of the specific microbiological profile in the context of this disease.
CONCLUSIONS: The study confirmed the hypothesis of an association between the microbiota composition and oral lichen planus, which may be of importance for the development of novel therapeutic approaches.
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About the authors
Natalia Pavlovna Teplyuk
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Email: teplyukn@gmail.com
ORCID iD: 0000-0002-5800-4800
SPIN-code: 8013-3256
MD, Dr. Sci. (Med.), Professor
Russian Federation, 8/2 Trubetskaya street, 119992 MoscowMikhail A. Stepanov
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Email: Doctor.stepanov@gmail.com
ORCID iD: 0000-0002-1872-9487
SPIN-code: 6524-5665
MD , Cand. Sci. (Med.), Assistant Professor
Russian Federation, 8/2 Trubetskaya street, 119992 MoscowBaira Sh. Damdinova
I.M. Sechenov First Moscow State Medical University (Sechenov University)
Author for correspondence.
Email: baira_d@mail.ru
ORCID iD: 0000-0002-4162-2928
Russian Federation, 8/2 Trubetskaya street, 119992 Moscow
Stepan V. Toshchakov
National Research Centre "Kurchatov Institute"
Email: stepan.toshchakov@gmail.com
ORCID iD: 0000-0001-7549-3450
SPIN-code: 8994-5224
National Research Centre "Kurchatov Institute"
Russian FederationSergey A. Noskov
Национальный исследовательский центр «Курчатовский институт»
Email: sergey.noskov.2001@icloud.com
ORCID iD: 0009-0006-1578-1382
National Research Centre "Kurchatov Institute"
Russian Federation, МоскваReferences
Supplementary files
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