Development and validation of an immunochromatographic test system for the determination of microcystins in water and tissues of freshwater fish

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Abstract

Introduction. Certain genera of cyanobacteria produce hepatotoxins associated with the group of microcystins (MCs). During periods of water blooms, cases of poisoning and death of fish, birds, wild and domestic animals, as well as humans, associated with the consumption of water, blue-green algae or other aquatic organisms contaminated with MC, have been recorded. Timely detection of MC in water reservoirs, in drinking water and in hydrobionts is necessary for carrying out preventive measures to prevent poisoning of people and animals with microcystins.

Results. An immunochromatographic test system has been developed for the determination of MC in drinking water, fresh water and fish tissues. When using the visual method of recording the results, the detection limit of the test system for determining MC-LR was no more than 0.1 ng/ml for drinking water and no more than 0.5 ng/ml for river and lake water, which is lower than maximum allowable concentration for MC-LR established by the World Health Organization (WHO) and Rospotrebnadzor. When analyzing fish tissues, the detection limit of the test system was no more than 3 ng/g tissue. The use of an instrumental method for recording results led to a 2–3-fold decrease in the minimum detectable concentration of MC-LR. The test system allowed semi-quantitative determination of MC content in water and fish tissues and detected different types of MC and the structurally similar hepatotoxin nodularin with varying efficiency.

Limitations. The test system does not allow the detection of some types of MC, but it identifies the most widespread and dangerous types, including MC-LR.

Conclusion. The developed test system can be used to monitor the content of MC in drinking water, water from open reservoirs and in fish tissues.

Compliance with ethical standards. The study does not require of the conclusion of the biomedical ethics committee or other documents.

Authors contribution. All co-authors made an equal contribution to the research and preparation of the article for publication.

Conflict of interest. The author declare no conflict of interest.

Acknowledgment. The work was carried out in accordance with the research plans of the Federal State Unitary Enterprise “Scientific Center “Signal” and the Institute of Cell Biophysics of the Federal Research Center “PSCBI RAS”.

Received: December 18, 2023 / Accepted: May 5, 2024 / Published: June 28, 2024

About the authors

Evgeny K. Singov

Federal state unitary enterprise "Scientific Center "Signal""

Author for correspondence.
Email: 9652291422@list.ru
ORCID iD: 0009-0001-3977-4455

Candidate of Medical Sciences, Head of the Research Group, FSUE NC Signal, 107014, Moscow, Russian Federation

e-mail: 9652291422@list.ru

Russian Federation

Oleg S. Morenkov

Institute of Cell Biophysics, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

Email: morenkov_o@mail.ru
ORCID iD: 0000-0001-9614-5236

Doctor of Biological Sciences, Director of the Institute of Cell Biophysics of the Pushchinsky Scientific Center for Biological Research of the Russian Academy of Sciences, 142290, Moscow Region, Pushchino, Russian Federation

e-mail: morenkov_o@mail.ru

Russian Federation

Sergey V. Sipin

Federal state unitary enterprise "Scientific Center "Signal""

Email: reepper@mail.ru
ORCID iD: 0009-0008-0027-6994

Senior Researcher at NIG FSUE NC Signal, 107014, Moscow, Russian Federation

e-mail: reepper@mail.ru

Russian Federation

Veronika V. Vrublevskaya

Institute of Cell Biophysics, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”

Email: v_vrublevskaya@mail.ru
ORCID iD: 0000-0002-0993-7356

Senior Researcher at the Institute of Cell Biophysics of the Pushchinsky Scientific Center for Biological Research of the Russian Academy of Sciences, 142290, Moscow Region, Pushchino, Russian Federation

e-mail: v_vrublevskaya@mail.ru

Russian Federation

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Copyright (c) 2024 Singov E.K., Morenkov O.S., Sipin S.V., Vrublevskaya V.V.


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