Study of the influence of fillers on the wettibility and cytotoxicity of polysiloxane films for medical use

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Abstract

A method for synthesis of two new types of polysiloxane-based films is proposed: with introduced chitosan and with introduced sodium bicarbonate with its subsequent removal. It was found that the obtained films have a branched internal structure of channels suitable for further filling with biologically active substances. The study of the hydrophobicity of the obtained films, in comparison with pure polysiloxane films, showed an insignificant (no more than 6%) decrease in the equilibrium wetting edge angle, as well as equalization of its values from the outer and inner sides, compared with films without modification. A comprehensive in vitro study demonstrated the promising use of polysiloxane films with introduced chitosan, since they do not have a reliable toxic effect on the studied cells. Films of pure polysiloxane and polysiloxane with the use of sodium hydrogen carbonate had a toxic effect on the investigated cells and acidified the medium. All samples were found to be non-adhesive to cells.

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About the authors

А. S. Baikin

A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences

Author for correspondence.
Email: baikinas@mail.ru
Russian Federation, 119334, Moscow

E. O. Nasakina

A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences

Email: baikinas@mail.ru
Russian Federation, 119334, Moscow

G. А. Davydova

Institute of Theoretical and Experimental Biophysics of Russian Academy of Sciences

Email: baikinas@mail.ru
Russian Federation, 142290, Pushchino

M. А. Sudarchikova

A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences

Email: baikinas@mail.ru
Russian Federation, 119334, Moscow

A. A. Melnikova

A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences

Email: baikinas@mail.ru
Russian Federation, 119334, Moscow

K. V. Sergienko

A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences

Email: baikinas@mail.ru
Russian Federation, 119334, Moscow

S. V. Konushkin

A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences

Email: baikinas@mail.ru
Russian Federation, 119334, Moscow

M. A. Kaplan

A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences

Email: baikinas@mail.ru
Russian Federation, 119334, Moscow

M. A. Sevostyanov

A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences; All-Russian Research Institute of Phytopathology

Email: baikinas@mail.ru
Russian Federation, 119334, Moscow; 143050, Bolshiye Vyazemy, Moscow Region

A. G. Kolmakov

A.A. Baikov Institute of Metallurgy and Materials Science, Russian Academy of Sciences

Email: baikinas@mail.ru

Corresponding Member of the RAS

Russian Federation, 119334, Moscow

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Supplementary files

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2. Fig. 1. SEM image of a section of polysiloxane films: without the use of fillers [24] (a), made using sodium bicarbonate (b) [24], made using chitosan (c).

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3. Fig. 2. Determination of the contact angle of polysiloxane films: without filler (a), produced using sodium bicarbonate (b), produced using chitosan (c).

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4. Fig. 3. Metabolic activity of NCTC L929 cells according to the results of the MTT test during incubation for 24 hours with 3-day extracts from polysiloxane films: made using chitosan (sample 1) without the use of fillers (sample 2), made using sodium bicarbonate (sample 3).

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5. Fig. 4. Appearance of mesenchymal stem cells (MSC) from human dental pulp (clone Th44) during incubation on the material 24 hours after seeding. Samples of polysiloxane films: made using chitosan (sample 1), without fillers (sample 2), made using sodium bicarbonate (sample 3). Sample K – Control. Stained with SYTO 9 (a), Hoechst 33342 (b) and PI (c). Scale bar 100 μm.

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