Intravenous administration of heterodimeric phospholipase A2 HDP-1 disturbs cardiovascular system functions and biochemical composition of blood in anesthesiated rats
- Authors: Ismailova A.M.1, Severyukhina M.S.1,2, Shaykhutdinova E.R.1, Perepechenova N.A.1, Starkov V.G.1, Dyachenko I.A.1,2, Tsetlin V.I.1, Utkin Y.N.1
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Affiliations:
- Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
- the Federal State Budgetary Educational Institution of Higher Education “Russian Biotechnological University (ROSBIOTECH)”
- Issue: Vol 522, No 1 (2025)
- Pages: 317-323
- Section: Articles
- URL: https://rjsvd.com/2686-7389/article/view/686334
- DOI: https://doi.org/10.31857/S2686738925030019
- ID: 686334
Cite item
Abstract
Heterodimeric phospholipase A2 HDP-1 from the venom of Nikolsky viper consists of two non-covalently bound subunits – HDP-1P and HDP-1In, the first of which exhibits lipolytic activity, while the second does not. The subunits have close molecular masses and similar amino acid sequences. Administration of HDP-1 and HDP-1P to anesthetized rats is accompanied by prolonged hypotensive effects, the intensity of which depends on the dose. With the administration of HDP-1P, the effect develops more slowly than in the case of HDP-1 containing both subunits, while individual HDP-1In does not affect hemodynamic parameters. It was found using electrocardiography that HDP-1 and HDP-1P affect the functioning of the rat heart, causing arrhythmia, which may indicate their ability to affect the innervation of the heart. The administration of HDP-1 and its subunits also leads to changes in biochemical blood parameters, indicating their damaging effect on cells and internal organs.
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About the authors
A. M. Ismailova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: utkin@ibch.ru
Branch
Russian Federation, Moscow; PushcinoM. S. Severyukhina
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; the Federal State Budgetary Educational Institution of Higher Education “Russian Biotechnological University (ROSBIOTECH)”
Email: utkin@ibch.ru
Branch, Pushchino branch
Russian Federation, Moscow; Pushchino; PuschinoE. R. Shaykhutdinova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: utkin@ibch.ru
Branch
Russian Federation, Moscow; PushcinoN. A. Perepechenova
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: utkin@ibch.ru
Branch
Russian Federation, PushchinoV. G. Starkov
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: utkin@ibch.ru
Russian Federation, Moscow
I. A. Dyachenko
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; the Federal State Budgetary Educational Institution of Higher Education “Russian Biotechnological University (ROSBIOTECH)”
Email: utkin@ibch.ru
Branch, Pushchino branch
Russian Federation, Moscow; Pushchino; PushchinoV. I. Tsetlin
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Email: utkin@ibch.ru
Corresponding Member of the RAS
Russian Federation, MoscowYu. N. Utkin
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences
Author for correspondence.
Email: utkin@ibch.ru
Russian Federation, Moscow
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