Point mutations in the M-domainof PCID2 impair its functionin mRNA exportin Drosophila melanogaster

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Abstract

PCID2 protein is a component of the eukaryotic TREX-2 complex responsible for mRNA export from the nucleus to the cytoplasm. Previously, we showed that PCID2 of D. melanogaster is involved in specific mRNA recognition and identified key amino acids responsible for interaction with the RNA of the ras2 gene. In this work, we show that point mutations of these amino acids disrupt the interaction of the protein with cellular RNA and the export of polyA-containing mRNA from the nucleus to the cytoplasm in Drosophila cells.

About the authors

Y. А. Vdovina

Institute of Molecular Biology, Russian Academy of Sciences

Author for correspondence.
Email: d_dmitrieva@mail.ru
Russian Federation, Moscow

S. G. Georgieva

Institute of Molecular Biology, Russian Academy of Sciences

Email: d_dmitrieva@mail.ru

Academician of the RAS

Russian Federation, Moscow

D. V. Kopytova

Institute of Molecular Biology, Russian Academy of Sciences

Email: d_dmitrieva@mail.ru
Russian Federation, Moscow

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