SIMULATED MICROGRAVITY AND COCULTURING WITH HEMATOPOIETIIC CELLS OPPOSITELY MODULATE WNT SIGNALING IN MESENCHYMAL STROMAL CELLS

A. Y. Ratushnyy; Ратушный А. Ю.; E. A. Tyrina; Тырина Е. А.; L. B. Buravkova; Буравкова Л. Б.

doi:10.31857/S2686738922600844

SIMULATED MICROGRAVITY AND COCULTURING WITH HEMATOPOIETIIC CELLS OPPOSITELY MODULATE WNT SIGNALING IN MESENCHYMAL STROMAL CELLS

Authors: Ratushnyy A.Y.¹, Tyrina E.A.¹, Buravkova L.B.¹
Affiliations:
1. Institute of Biomedical Problems, Russian Academy of Sciences
Issue: Vol 510, No 1 (2023)
Pages: 268-272
Section: Articles
URL: https://rjsvd.com/2686-7389/article/view/651108
DOI: https://doi.org/10.31857/S2686738922600844
EDN: https://elibrary.ru/UNDBOY
ID: 651108

Cite item

Full Text

Open Access
Restricted Access

Access granted
Restricted Access

Subscription or Fee Access

Abstract

The osteogenic potential of mesenchymal stromal cells (MSCs) can determine the bone homeostasis and the physical characteristics of bones. Microgravity reduces the ability of these cells to differentiate in osteogenic direction. It has been shown that the addition of hematopoietic stem and progenitor cells (HSPCs) to MSC culture in vitro can have the opposite effect. The aim of this study was to identify transcriptional changes in 84 genes associated with Wnt signaling in MSCs during microgravity simulation and interaction with HSPCs. The results indicate an increase in the non-canonical Wnt signaling activity during MSCs and HSPCs cocultivation, while simulated microgravity enhances the canonical component of this signaling pathway. These changes may underlie the modulation of osteogenic potential of MSCs in hematopoietic niche under microgravity.

Keywords

mesenchymal stromal cells (MSCs), simulated microgravity, Wnt signaling

References

Buravkova L.B., Gershovich P.M., Gershovich J.G., et al. Mechanisms of gravitational sensitivity of osteogenic precursor cells //Acta Naturae (англоязычная версия). 2010. V. 2. № 1 (4). P. 28–35.
Man J., Graham T., Squires-Donelly G., et al. The effects of microgravity on bone structure and function // npj Microgravity. 2022. V. 8. № 1. P. 1–15.
Andreeva E.R., Ezdakova M.I., Bobyleva P.I., et al. Osteogenic Commitment of MSC Is Enhanced after Interaction with Umbilical Cord Blood Mononuclear Cells In Vitro //Bulletin of Experimental Biology and Medicine. 2021. V. 171. № 4. P. 541–546.
Jia Y., Zhang C., Zheng X., et al. Co-cultivation of progenitor cells enhanced osteogenic gene expression and angiogenesis potential in vitro // Journal of International Medical Research. 2021. V. 49. № 4. P. 03000605211004024.
Yang X., Sun L.W., Liang M., et al. The response of wnt/ß-catenin signaling pathway in osteocytes under simulated microgravity // Microgravity Science and Technology. 2015. V. 27. № 6. P. 473–483.
Houschyar K.S., Tapking C., Borrelli M.R., et al. Wnt pathway in bone repair and regeneration–what do we know so far //Frontiers in cell and developmental biology. 2019. V. 6. P. 170.
Takam Kamga P., Bazzoni R., Dal Collo G., et al. The role of notch and Wnt signaling in MSC communication in normal and leukemic bone marrow niche // Frontiers in cell and developmental biology. 2021. V. 8. P. 599276.
Dominici M., Le Blanc K., Mueller I., et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement //Cytotherapy. 2006. V. 8. № 4. P. 315–317.
Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2– ΔΔCT method //methods. 2001. V. 25. № 4. P. 402–408.
Gu Q., Tian H., Zhang K., et al. Wnt5a/FZD4 mediates the mechanical stretch-induced osteogenic differentiation of bone mesenchymal stem cells //Cellular Physiology and Biochemistry. 2018. V. 48. № 1. P. 215–226.
Yang X., Sun L.W., Liang M., et al. The response of wnt/ß-catenin signaling pathway in osteocytes under simulated microgravity //Microgravity Science and Technology. 2015. V. 27. № 6. P. 473–483.
Jothimani G., Di Liddo R., Pathak S., et al. Wnt signaling regulates the proliferation potential and lineage commitment of human umbilical cord derived mesenchymal stem cells //Molecular Biology Reports. 2020. V. 47. № 2. P. 1293–1308.
Albers J., Keller J., Baranowsky A., et al. Canonical Wnt signaling inhibits osteoclastogenesis independent of osteoprotegerin //Journal of Cell Biology. 2013. V. 200. № 4. P. 537–549.
Ono M., Inkson C.A., Kilts T.M., et al. WISP-1/CCN4 regulates osteogenesis by enhancing BMP-2 activity // Journal of Bone and Mineral Research. 2011. V. 26. №. 1. P. 193–208.