SIMULATED MICROGRAVITY AND COCULTURING WITH HEMATOPOIETIIC CELLS OPPOSITELY MODULATE WNT SIGNALING IN MESENCHYMAL STROMAL CELLS

A. Y. Ratushnyy; Ратушный А. Ю.; E. A. Tyrina; Тырина Е. А.; L. B. Buravkova; Буравкова Л. Б.

doi:10.31857/S2686738922600844

SIMULATED MICROGRAVITY AND COCULTURING WITH HEMATOPOIETIIC CELLS OPPOSITELY MODULATE WNT SIGNALING IN MESENCHYMAL STROMAL CELLS

Authors: Ratushnyy A.Y.¹, Tyrina E.A.¹, Buravkova L.B.¹
Affiliations:
1. Institute of Biomedical Problems, Russian Academy of Sciences
Issue: Vol 510, No 1 (2023)
Pages: 268-272
Section: Articles
URL: https://rjsvd.com/2686-7389/article/view/651108
DOI: https://doi.org/10.31857/S2686738922600844
EDN: https://elibrary.ru/UNDBOY
ID: 651108

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Abstract
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Abstract

The osteogenic potential of mesenchymal stromal cells (MSCs) can determine the bone homeostasis and the physical characteristics of bones. Microgravity reduces the ability of these cells to differentiate in osteogenic direction. It has been shown that the addition of hematopoietic stem and progenitor cells (HSPCs) to MSC culture in vitro can have the opposite effect. The aim of this study was to identify transcriptional changes in 84 genes associated with Wnt signaling in MSCs during microgravity simulation and interaction with HSPCs. The results indicate an increase in the non-canonical Wnt signaling activity during MSCs and HSPCs cocultivation, while simulated microgravity enhances the canonical component of this signaling pathway. These changes may underlie the modulation of osteogenic potential of MSCs in hematopoietic niche under microgravity.

Keywords

mesenchymal stromal cells (MSCs), simulated microgravity, Wnt signaling

References

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