Biological and Photocatalytic Activities of Silver Nanoparticles Synthesized from the Leaf Extract of Euphorbia royleana Boiss


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Abstract

Introduction:Silver nanoparticles (AgNPs) have gained significance due to their practical use in the medicinal field, especially in the treatment of tumors and cancer. The current article explores a green synthetic method for the preparation of AgNPs using leaf extract of Euphorbia royleanas.

Methods:The synthesis was conducted at different parameters like concentration of AgNO3, pH, salt concentration, temperature and time to optimize best results for their biochemical applications. It was validated through UV-V spectroscopy (400-450 nm) with 1:3 (concentration ratio of leaf ethanolic extract and 1 mM AgNO3 solution) at a pH value of 8 at 35oC, which were the best optimization conditions. The FTIR spectral bands showed the presence of C-N and –OH functional groups, indicating that –OH stretching and the aliphatic -C-H stretching were involved in the reduction of Ag ions. The XRD pattern showed the face-centered cubic structure of silver nanoparticles. The results of SEM revealed that AgNPs were predominantly spherical in shape, mono-dispersed, and arranged in scattered form. EDX analysis testified the presence of metallic silver along with other elements like Cl, C, and O.

Results:The investigation of biochemical parameters showed that AgNPs were influential in the discoloration of dye wastewater (methylene blue ), where 80% of dye color was removed in 20 min, followed by the significant (p < 0.05) analgesic activity with an inhibition percentage of 86.45% at a dose of 500 mg/kg.

Conclusion:Similarly, the antioxidant activity with the highest percent inhibition was 55.4% (p < 0.0001), shown by the AgNPs at 500 µg/mL. AgNPs showed a 30 mm zone of inhibition at 100 µl/mL against Aspergillus niger. It was concluded that AgNPs provide a baseline in medical technology for the treatment of simple to chronic diseases.

About the authors

Hussan Ara Begum

Department of Botany, Abdul Wali Khan University Mardan

Email: info@benthamscience.net

Sana Yaseen

Department of Botany, Abdul Wali Khan University Mardan

Email: info@benthamscience.net

Amir Zada

Department of Chemistry, Abdul Wali Khan University Mardan

Email: info@benthamscience.net

Muhammad Musa

Department of Botany, Abdul Wali Khan University Mardan

Email: info@benthamscience.net

Asif Khan

Department of Pharmacy, State University of Maringa

Email: info@benthamscience.net

Sheheryar

Department of Animal Sciences, Federal University of Ceará

Email: info@benthamscience.net

Abdulwahed Alrefaei

Department of Zoology, College of Science, King Saud University

Email: info@benthamscience.net

Mikhlid Almutairi

Department of Zoology, College of Science, King Saud University

Email: info@benthamscience.net

Sajid Ali

Department of Horticulture and Life Science, Yeungnam University

Author for correspondence.
Email: info@benthamscience.net

Rafia Azmat

Department of Chemistry, University of Karachi

Email: info@benthamscience.net

Muhammad Hamayun

Department of Botany, Abdul Wali Khan University Mardan

Author for correspondence.
Email: info@benthamscience.net

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