The content of endogenous hormones in explants and calluses of Lavandula angustifolia Mill. at the initial stages of in vitro culture

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The content of endogenous hormones (auxin IAA, cytokinines, ABA) in explants of various types (segments of leaf, bud, stem), primary calluses induced from them, as well as morphogenic and non-morphogenic calluses at the initial stages of in vitro culture by the immunoassay method was studied for the first time for Lavandula angustifolia Mill. The maximum value of hormone levels in such explants as segments of bud was shown. An increase in the content of hormones in primary calluses was revealed in comparison with similar characteristics in all types of explants. The higher level of the active form of cytokinin (trans-zeatin) and ABA, as well as the lower level of the inactive form of cytokinin (zeatin-nucleotide) and auxin IAA were identified in morphogenic callus compared with non-morphogenic callus. It is suggested that the content of endogenous hormones in explants and calluses of L. angustifolia is due to their histological status. The conclusion is made about the unified histophysiological mechanisms of callusogenesis and morphogenesis in vitro in the studied plant.

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N. N. Kruglova

Research Institute of Agriculture of Crimea; Ufa Institute of biology – subdivision of the UFRC RAS

Author for correspondence.
Email: kruglova@anrb.ru
Russian Federation, Kievskaya str., 150, Simferopol, 295043; pr. Oktyabrya, 69, Ufa, 450054

I. R. Galin

Ufa Institute of biology – subdivision of the UFRC RAS

Email: kruglova@anrb.ru
Russian Federation, pr. Oktyabrya, 69, Ufa, 450054

N. A. Yegorova

Research Institute of Agriculture of Crimea

Email: kruglova@anrb.ru
Russian Federation, Kievskaya str., 150, Simferopol, 295043

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2. Fig. 1. Explants (a), primary calli (b), non-morphogenic (c) and morphogenic (d) calli of the first passage of L. angustifolia obtained from different explants. Legend: B – bud, PK – primary callus, SL – leaf segment, SP – bud segment, SS – stem segment. Scale: 10 mm.

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3. Fig. 2. Content of endogenous hormones in various explants of L. angustifolia: a – IAA, b – ABA, c – cytokinins.

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4. Fig. 3. Content of endogenous hormones in primary calli of L. angustifolia obtained from different explants: a – IAA, b – ABA, c – cytokinins.

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5. Fig. 4. Content of endogenous hormones in morphogenic and non-morphogenic calli of the first passage of L. angustifolia, obtained from different explants: a – IAA, b – ABA, c – cytokinins. Note: data on the content of endogenous hormones in morphogenic calli of the first passage, obtained from the stem, are not available, explanation in the text.

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