Effect of copper oxide nanoparticles on gene expression of NMDA receptor

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Abstract

Introduction. Copper plays an important role in the metabolism of the brain, but particles of copper, in the nanometer range, exhibit neurotoxic properties and cause malfunctioning of brain cells.

Material and methods. For 6 weeks, 3 times a week, the animals were injected with a suspension of NPs of copper oxide. The determination of the expression of the genes GRIN1, GRIN2a, and GRIN2b, encoding the proteins GluN1, GluN2a, and GluN2b, respectively, was carried out by real-time PCR with probes.

Results. A statistically significant decrease in the expression level of genes encoding NMDA receptor proteins was determined when exposed to 0.5 mg/ml CuO nanoparticles (ΔCt(GRIN1) = 0.813; ΔCt(GRIN2A) = 3.477; ΔCt(GRIN2B) = 1.37) in comparison with control group (ΔCt(GRIN1) = 6.301; ΔCt(GRIN2A) = 7.823; ΔCt(GRIN2B) = 4.747).

Conclusion. Evaluation of gene expression of the NMDA receptor may be present in a genetic marker to determine the toxic effect of copper oxide nanoparticles; however, further studies are needed, including behavioral tests to confirm the clinical manifestations of neurodegenerative disorders.

About the authors

Ivan Andreevich Sitnikov

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Author for correspondence.
Email: sitnikovia@ymrc.ru
ORCID iD: 0000-0002-4109-9268

Junior Researcher, Department of Molecular Biology and Electron Microscopy, FBRI YMRC PHPIW of Rospotrebnadzor, 620014, Yekaterinburg, Russia.

e-mail: sitnikovia@ymrc.ru

Russian Federation

Daria Ramilevna Shaikhova

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: darya.boo@mail.ru
ORCID iD: 0000-0002-7029-3406

Младший научный сотрудник отдела молекулярной биологии и электронной микроскопии ФБУН «Екатеринбургский медицинский научный центр профилактики и охраны здоровья рабочих промпредприятий» Роспотребнадзора, 620014, г. Екатеринбург

e-mail: darya.boo@mail.ru

Russian Federation

Anna Mikhailovna Amromina

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: amrominaam@ymrc.ru
ORCID iD: 0000-0001-8794-7288

Младший научный сотрудник отдела молекулярной биологии и электронной микроскопии ФБУН «Екатеринбургский медицинский научный центр профилактики и охраны здоровья рабочих промпредприятий» Роспотребнадзора, 620014, г. Екатеринбург

e-mail: amrominaam@ymrc.ru

Russian Federation

Marina Petrovna Sutunkova

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: sutunkova@ymrc.ru
ORCID iD: 0000-0002-1743-7642

Доктор медицинских наук, директор ФБУН «Екатеринбургский медицинский научный центр профилактики и охраны здоровья рабочих промпредприятий» Роспотребнадзора, 620014, г. Екатеринбург

e-mail: sutunkova@ymrc.ru

Russian Federation

Yuliya Vladimirovna Ryabova

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: ryabova@ymrc.ru
ORCID iD: 0000-0003-2677-0479

Младший научный сотрудник отдела токсикологии и биопрофилактики ФБУН «Екатеринбургский медицинский научный центр профилактики и охраны здоровья рабочих промпредприятий» Роспотребнадзора, 620014, г. Екатеринбург

e-mail: ryabova@ymrc.ru

Russian Federation

Anastasiya Valerevna Tazhigulova

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: vasilyevaav@ymrc.ru
ORCID iD: 0000-0001-9384-8550

Лаборант-исследователь отдела токсикологии и биопрофилактики ФБУН «Екатеринбургский медицинский научный центр профилактики и охраны здоровья рабочих промпредприятий» Роспотребнадзора, 620014, г. Екатеринбург

e-mail: vasilyevaav@ymrc.ru

Russian Federation

Vadim Olegovich Ruzakov

Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers

Email: ruzakov@ymrc.ru
ORCID iD: 0000-0002-8902-0416

Помощник директора по развитию ФБУН «Екатеринбургский медицинский научный центр профилактики и охраны здоровья рабочих промпредприятий» Роспотребнадзора, 620014, г. Екатеринбург

 e-mail: ruzakov@ymrc.ru

Russian Federation

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Copyright (c) 2021 Sitnikov I.A., Shaikhova D.R., Amromina A.M., Sutunkova M.P., Ryabova Y.V., Tazhigulova A.V., Ruzakov V.O.


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