Email this article ACTIVITY OF APOPTOSIS IN THE NERVE TISSUE OF WHITE RATS EXPOSED TO NANOSILVER ARABINOGALACTAN
- Authors: Sosedova L.M.1, Kapustina E.A.1, Novikov M.A.1
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Affiliations:
- East-Siberian Institute of Medico-Ecological Researches
- Issue: No 6 (2015)
- Pages: 27-31
- Section: Articles
- Published: 04.12.2015
- URL: https://rjsvd.com/0869-7922/article/view/641360
- DOI: https://doi.org/10.15690/vramn522
- ID: 641360
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Abstract
The article presents results of apoptosis evaluation in albino rat neurons using comet assay for DNA and immune histochemical analysis to determine the activity of pro-and anti-apoptotic proteins caspase 3 and bcl-2 at exposure to nano biocomposites representing nano silver embedded in a matrix of a natural arabinogalactan. In preparations of nervous tissue of rats exposed to nano silver arabinogalactan polymer, a statistically significant increase of DNA content in comet tails was found out. DNA comets with DNA tail content of more than 70%, which could be regarded as apoptotic have not been observed in investigated formulations. The results of the immune histochemical determination of expression of anti-and pro- apoptotic proteins bcl-2 and caspase-3 in neurons show on the contrary the development of apoptosis in nerve cells. Comparing results of the studies performed, a conclusion can be drawn that to detect the apoptosis process, immune histochemical methods for determination of anti-and pro-apoptotic proteins are more efficient than DNA comet assay.
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About the authors
Larisa Mikhailovna Sosedova
East-Siberian Institute of Medico-Ecological Researches
Author for correspondence.
Email: noemail@neicon.ru
Д.м.н., проф., зав.лабораторией биомоделирования и трансляционной медицины ФГБНУ «Восточно-Сибирский институт медико-экологических исследований», 665827, г. Ангарск, Российская Федерация,
e-mail: sosedlar@mail.ru
Russian FederationEkaterina Aleksandrovna Kapustina
East-Siberian Institute of Medico-Ecological Researches
Email: noemail@neicon.ru
К.м.н., научный сотрудник лаборатории биомоделирования и трансляционной медицины ФГБНУ «Восточно-Сибирский институт медико-экологических исследований», 665827, г. Ангарск, Российская Федерация
e-mail: tox_lab@mail.ru
Russian FederationMikhail Aleksandrovich Novikov
East-Siberian Institute of Medico-Ecological Researches
Email: noemail@neicon.ru
М.н.с., лаборатории биомоделирования и трансляционной медицины ФГБНУ «Восточно-Сибирский институт медико-экологических исследований», 665827, г. Ангарск, Российская Федерация
e-mail: novik-imt@mail.ru
Russian FederationReferences
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