Email this article THE STUDY OF ATROPINE EFFECT ON THE BLOOD ESTERASE STATUS IN RATS AT DIFFERENT WAYS OF UPTAKE OF MALATHION BY THE ORGANISM
- Authors: Yudin M.A.1, Bykov V.N.1, Kolesnikov A.M.1, Sarana A.M.2, Yudnikov I.V.1
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Affiliations:
- Scientific Research Test Institute of Military Medicine, S.M. Kirov Medical Military Academy, Ministry of Defense
- City Hospital № 40
- Issue: No 3 (2015)
- Pages: 16-20
- Section: Articles
- Published: 12.06.2015
- URL: https://rjsvd.com/0869-7922/article/view/641306
- DOI: https://doi.org/10.15690/vramn454
- ID: 641306
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Abstract
The therapeutic action of atropine (0.4, 2, 20 mg/kg) was assessed on models of rats intramuscular and intragastric poisoning with malathion in a dose of 1 LD50. It was demonstrated that the drug prevented animals death after the intramuscular administration of malathion and promoted the reduction of intensity of a cholinopositive symptomatology. It also promoted the recovery of the acetyl cholinesterase activity in rats blood up to 80% 8 hours after the therapy, and 24 hours after the injection of malathion, this index reached levels in the control group. The preparation had no protective effect on the modelled intragastric poisoning by malathion independently of its dose. In animals to which atropine (2mg/kg) was administrated an hour after intoxication, the enzyme activity in blood was 40% while in the control group it was 80%. It is practicable to take these findings into consideration while searching and selecting efficient medications for emergency treatment of organophosphorous intoxications.
About the authors
Mikhail Anatol’evich Yudin
Scientific Research Test Institute of Military Medicine, S.M. Kirov Medical Military Academy, Ministry of Defense
Author for correspondence.
Email: noemail@neicon.ru
Кандидат медицинских наук, доцент, заместитель начальника отдела НИИИ (ВМ) ВМедА им. С.М. Кирова, 195043, г. Санкт-Петербург, Российская Федерация
e-mail: mihei_bridge@mail.ru
Russian FederationVladimir Nikolaevich Bykov
Scientific Research Test Institute of Military Medicine, S.M. Kirov Medical Military Academy, Ministry of Defense
Email: noemail@neicon.ru
Доктор медицинских наук, профессор, начальник НИИЦ (МБЗ) НИИИ (ВМ) ВМедА им. С.М. Кирова, 195043, г. Санкт-Петербург, Российская Федерация
e-mail: ykov_imm@mail.ru
Russian FederationAlexandr Maratovich Kolesnikov
Scientific Research Test Institute of Military Medicine, S.M. Kirov Medical Military Academy, Ministry of Defense
Email: noemail@neicon.ru
Лаборант-исследователь НИИИ (ВМ) ВМедА им. С.М. Кирова, 195043, г. СанктПетербург, Российская Федерация
e-mail: Alex9_6_89@mail.ru
Russian FederationAndrey Mikhaylovic Sarana
City Hospital № 40
Email: noemail@neicon.ru
Кандидат медицинских наук, доцент, заместитель главного врача по реабилитации СПб ГБУЗ «Городская больница № 40», главный специалист по медицинской реабилитации и санитарно-курортному делу Комитета по Здравоохранению
правительства г. Санкт-Петербурга, 197706, г. Сестрорецк, г. Санкт-Петербург, Российская Федерация
Il’ya Vladimirovich Yudnikov
Scientific Research Test Institute of Military Medicine, S.M. Kirov Medical Military Academy, Ministry of Defense
Email: noemail@neicon.ru
Научный сотрудник НИИИ (ВМ) ВМедА им. С.М. Кирова, 195043, г. Санкт-Петербург, Российская Федерация
Russian FederationReferences
- Biderman F. M. Intensive therapy under acute malathion poisonings at a preclinical stage: Doctoral dissertation thesis / F. M. Biderman, SPb 1991 LGIUV named by S. M. Kirov - 286 p. (in Russian)
- Mironov A. N. Manual for preclinical research studies of medicinalpreparations. Part I. M., 2012 (in Russian)
- Sosyukin A. E. Detoxification therapy of acute organophosphorous compound poisonings. Doctoral dissertation thesis / A. E. Sosyukin SPb 1992 Kirov Military Medical Academy - 333 p.(in Russian)
- Ellman G.L. A new and rapid colometric determination of acethylcholinesterases activity / G.L.Ellman, K.D.Courtney, V.Andres [et al.] // Biochem. pharmacol.-1961.-Vol. 7.-P.88-95.
- Gupta C. Handbook of toxicology of chemical warfare agents / C. Gupta // Elsevier London. - 2009.
- Isenring R. Pesticides reduce biodiversity / R. Isenring // Pesticides news - 2010. - No 88
- Jokanovic M. Organophosphate Induced Delayed Polyneuropathy / M. Jokanovic, P. V. Stukalov, M. Kosanovic // Toxicol Lett, N 190 (2) ( 2009).
- Kuka K. Pralidoxime - the gold standard of acetylcholinesterase reactivators -reactivation in vitro efficacy / K. Kuka, M. Hrabinova, O. Soukup [et al.], Bratisl. Lek. Listy, Vol. 111, N. 9, P 502 - 504 (2010).
- Milatovic D. Carbofuran-induced oxidative stress in slow and fast skeletal muscles: prevention by memantine and atropine / D. Milatovic, R.C. Gupta, A. Dekundy, [et al.] //Toxicology. 2005. Vol 208, №1. - P 13-24.
- Musilova L. In vitro oxime-assisted reactivation of paraoxon-inhibited human acetylcholinesterase and butyrylcholinesterase / L. Musilova, K. Kuca, Y. Jung [et al.], Clin. Toxicol. (Phila), Vol. 47, P. 545 - 550 (2009).
- Vitorovic-Todorovic M.D. Structural modifications of 4-aryl-4-oxo-2-aminylbutanamides and their acetyl- an dbutyrylcholinesterase inhibitory activity. Investigation of AChE-ligand interactions by docking calculations and molecular dynamics simulations / M.D. Vitorovic-Todorovic, C. Koukoulitsa, I.O. Juranic [et al.] // Eur J Med Chem. 2014 May 4;81C:158-175.
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