Email this article EXPERIMENTAL STUDY OF ALLERGENIC PROPERTIES AND IMMUNOTOXICITY OF THE DRUG STODAL
- Authors: Kovalenko L.P.1, Tallerova A.V.1, Kuznetsova O.S.1, Lapitskaya A.S.1
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Affiliations:
- Federal State Budgetary Institution «V.V. Zakusov research Institute of Pharmacology» under the Russian Academy of Medical research Science
- Issue: No 2 (2015)
- Pages: 26-30
- Section: Articles
- Published: 06.04.2015
- URL: https://rjsvd.com/0869-7922/article/view/641303
- DOI: https://doi.org/10.15690/vramn445
- ID: 641303
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Abstract
Allergenicity and immunotoxicity of Stodal preparation was assessed. Immunization of guinea pigs with Stodal in doses of 1 ml/kg and 10 ml/kg according to a standard scheme or mixed with complete Freund’s adjuvant did not provoke a systemic anaphylactic reaction or delayed allergenic reactions in animals. A single per os administration of the Stodal preparation in doses of 1mg/kg and 10 ml/kg to CBA line mice did not affect the inflammation response to concanavalin A (Con.A).
In F1 (CBA x C57BL/6) mice , Stodal perorally administrated at a dose of 1 ml/mg over 14 days caused a significant increase of thymus mass index and cellularity by 41.5% and in a dose of 10 ml/kg the thymus mass increased by 28.7% . The preparation administrated in the doses of 1 ml/kg and 10 ml/kg did not affect peritoneal macrophage phagocytic activity and indicators of the neutrophils hemi-luminescent response to opsonized zymosan. A two week administration of Stodal preparation in doses of 1 ml/kg and 10 ml/kg to CBA and C57BL/6 strain mice induced a significant stimulation of the humoral immune response. In F1 (CBA x C57BL/6) hybrid mice , 1 ml/kg dose administration induced an authentic increase of cellular immunity indicators. Thus, the Stodal preparation in the dose range investigated does not produce allergenic responses of non-delayed and delayed types and pseudoallergic reactions, stimulates humoral and cellular immunity and does not produce immune-toxic effect.
About the authors
Larisa Petrovna Kovalenko
Federal State Budgetary Institution «V.V. Zakusov research Institute of Pharmacology» under the Russian Academy of Medical research Science
Author for correspondence.
Email: noemail@neicon.ru
Доктор биологических наук, ведущий научный сотрудник лаборатории лекарственной токсикологии Федерального государственного бюджетного учреждения «Научно-исследовательского института фармакологии имени В.В. Закусова»
Российской академии медицинских наук, г. Москва
e-mail: kovalenko.larisa@mail.ru
Russian FederationAnna Vadimovna Tallerova
Federal State Budgetary Institution «V.V. Zakusov research Institute of Pharmacology» under the Russian Academy of Medical research Science
Email: noemail@neicon.ru
Кандидат биологических наук, старший научный сотрудник лаборатории лекарственной токсикологии Федерального государственного бюджетного учреждения «Научно-исследовательского института фармакологии имени В.В. Закусова»
Российской академии медицинских наук, г. Москва
e-mail: annatall@rambler.ru
Russian FederationOlga Sergeevna Kuznetsova
Federal State Budgetary Institution «V.V. Zakusov research Institute of Pharmacology» under the Russian Academy of Medical research Science
Email: noemail@neicon.ru
Младший научный сотрудник сотрудник лаборатории лекарственной токсикологии Федерального государственного бюджетного учреждения «Научно-исследовательского института фармакологии имени В.В. Закусова» Российской
академии медицинских наук, г. Москва
e-mail: olgapharm@inbox.ru
Russian FederationAnastasya Sergeevna Lapitskaya
Federal State Budgetary Institution «V.V. Zakusov research Institute of Pharmacology» under the Russian Academy of Medical research Science
Email: noemail@neicon.ru
Кандидат биологических наук, старший научный сотрудник лаборатории фармакологической генетики Федерального государственного бюджетного учреждения «Научно-исследовательского института фармакологии имени В.В. Закусова» Российской академии медицинских наук, г. Москва
e-mail: lapitskaia.n@mail.ru
Russian FederationReferences
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