STUDY OF TOXICITY AND SAFETY OF LIPOPHILIC EXTRACTS OF THE WHITE SEA BROWN ALGAE – FUCUS VESICULOSUS AND LAMINARIA SACCHARINA ON THE DAPHNIA MAGNA STRAUS MODEL

Assessment of the acute toxicity, safety and biological activity of lipophilic extracts of the White Sea brown algae“– S. latissima and F. vesiculosus– on the model of zoohydrobionts Daphnia magna Straus has been performed.

Two methods of obtaining dry lipid concentrate of kelp and fucus were tested. It has been shown that the acute toxicity of the lipid extract of kelp does not depend on the method of its preparation and is about 200 mg/L. The acute toxicity of fucus extract depends on the method of preparation. It is about 100 mg/L in the «cold» method of extraction and increases by an order of magnitude in the «hot» method. To study the biological activity, samples of brown algae obtained by «cold» extraction were used.

In chronic experiments, preparations of brown algae were used in a concentration of 8.0 mg/L, which was less than 0,1 LC50 of the acute toxicity of fucus and did not cause toxic effects.

For 24 days, the preparations were introduced in contact with Daphnia twice a week. Subsequently, the control and experimental hydrobionts were kept under the same conditions until the death of the last individuals. It has been shown that the preparations of S. latissima and F. vesiculosus in the proposed concentration by 20-30% increased the lifespan of Daphnia in normal conditions and by 50-60% in extreme conditions, while stimulating the reproductive activity of aquatic organisms by 3-4 times. The results obtained suggest that the studied drugs have pronounced adaptogenic and cytoprotective effects.