Email this article ANALYSIS OF MT2A AND MT3 GENE EXPRESSION IN RAT'S LIVER AND KIDNEY IN RESPONSE TO CADMIUM CHLORIDE POISONING
- Authors: Ziatdinova M.M.1, Yakupova T.G.1, Valova Y.V.1, Mukhammadieva G.F.1, Karimov D.O.1, Nazarova L.S.1, Smolyankin D.A.1
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Affiliations:
- Ufa Research Institute of Occupational Medicine and Human Ecology
- Issue: No 6 (2020)
- Pages: 38-42
- Section: Articles
- Published: 04.01.2021
- URL: https://rjsvd.com/0869-7922/article/view/641254
- DOI: https://doi.org/10.36946/0869-7922-2020-6-38-42
- ID: 641254
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Abstract
The aim of this study was to investigate the expression of metallothionein genes in the liver and kidneys of rats with acute cadmium poisoning.
Simulation of poisoning with cadmium chloride was carried out on white outbred female rats, divided into 4 groups depending on the dose of the injected toxicant. RNA samples isolated from rat liver and kidneys were used as research materials.
The multiplicity of expression of the MT3 gene in the kidneys increased at the lowest dose of CdCl2 , which was used in this experiment (0.029 mg / kg); with increasing dosage, the expression level decreased, but not lower than the control values. Analysis of the expression of the same gene in the liver showed a tendency towards a decrease in the content of transcripts with increasing dose. The frequency of expression of the MT2A gene at higher doses of CdCl2 increased both in the liver and in the kidneys.
In the present work, statistically significant dose-dependent changes in the expression multiplicity of metallothionein genes were detected 24 hours after CdCl2 administration. The revealed differences in the level of transcriptional activity of metallothionein genes require further investigation, since there are probably differences in the level of gene expression at earlier and later periods of toxicant action.
About the authors
M. M. Ziatdinova
Ufa Research Institute of Occupational Medicine and Human Ecology
Author for correspondence.
Email: munira.munirovna@yandex.ru
Ziatdinova Munira Munirovna
450106, Ufa
Russian FederationT. G. Yakupova
Ufa Research Institute of Occupational Medicine and Human Ecology
Email: tanya.kutlina92@mail.ru
Yakupova Tatyana Georgievna
450106, Ufa
Russian FederationYa. V. Valova
Ufa Research Institute of Occupational Medicine and Human Ecology
Email: fake@neicon.ru
Valova Yana Valer’evna
450106, Ufa
Russian FederationG. F. Mukhammadieva
Ufa Research Institute of Occupational Medicine and Human Ecology
Email: ufniimt@mail.ru
Mukhammadieva Guzel Fanisovna
450106, Ufa
Russian FederationD. O. Karimov
Ufa Research Institute of Occupational Medicine and Human Ecology
Email: karimovdo@gmail.com
Karimov Denis Olegovich
450106, Ufa
Russian FederationL. Sh. Nazarova
Ufa Research Institute of Occupational Medicine and Human Ecology
Email: lilinaz19@mail.ru
Nazarova Liliya Shamilevna
450106, Ufa
Russian FederationD. A. Smolyankin
Ufa Research Institute of Occupational Medicine and Human Ecology
Email: smolyankin.denis@yandex.ru
Smolyankin Denis Anatol’evich
450106, Ufa
Russian FederationReferences
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