Protective influence of coded amino acids on the development of liver tissue culture in the presence of cytostatic

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Abstract

An urgent problem is the search for substances that can provide a protective effect in cases of DNA synthesis and repair disorders that arise as a result of side effects of cytostatic drugs used in the treatment of cancer. The aim of this work was to study the effect of 20 encoded amino acids in the presence of Cyclophosphane on the development of organotypic culture of rat liver tissue. The results obtained indicate that Cyclophosphane; which simulates the action of such cytostatic substances; inhibits cell proliferation in the liver tissue. It was also found that the encoded amino acids: asparagine; arginine; and glutamic acid; eliminate the inhibitory effect of Cyclophosphane in liver tissue culture. The growth zone of explants after combined exposure to Cyclophosphane (whose isolated action suppressed the growth zone) and these amino acids increased significantly and reached control values. Thus; the experimental data create the basis for the development of methods for the therapeutic use of the three studied amino acids for the removal of side effects in the treatment with cytostatic drugs.

About the authors

N. I. Chalisova

Pavlov Institute of Physiology; Russian Academy of Sciences

Author for correspondence.
Email: ni_chalisova@mail.ru

Chalisova Natalia Iosifovna

199034; Saint Petersburg

Russian Federation

V. K. Kozlov

Institute of Toxicology of the Federal Medical Biological Agency

Email: kvk52@mail.ru

Kozlov Victor Konstantinovich

192019; Saint Petersburg

Russian Federation

A. B. Mulik

Institute of Toxicology of the Federal Medical Biological Agency

Email: mulikab@mail.ru

Mulik Alexander Borisovich

192019; Saint Petersburg

Russian Federation

E. P. Zatsepin

Institute of Toxicology of the Federal Medical Biological Agency

Email: institute@toxicology.ru

Zatsepin Eduard Pavlovich

192019; Saint Petersburg

Russian Federation

T. A. Kostrova

Institute of Toxicology of the Federal Medical Biological Agency

Email: tasia.pcma.le4@mail.ru

Kostrova Taisiya Alexandrovna

192019; Saint Petersburg

Russian Federation

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Copyright (c) 2020 Chalisova N.I., Kozlov V.K., Mulik A.B., Zatsepin E.P., Kostrova T.A.


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