Synthesis of Fluorescent-Labeled Nucleotides for Labeling of Isothermal Amplification Products

V. E. Shershov; Шершов В. Е.; V. E. Kuznetsova; Кузнецова В. Е.; A. S. Zasedatelev; Заседателев А. С.; V. A. Vasiliskov; Василисков В. А.; E. Yu. Shekalova; Шекалова Е. Ю.; G. F. Shtylev; Штылев Г. Ф.; I. Yu. Shishkin; Шишкин И. Ю.; A. I. Levashova; Левашова А. И.; S. A. Lapa; Лапа С. А.; A. V. Chudinov; Чудинов А. В.

doi:10.31857/S0132342323050056

Synthesis of Fluorescent-Labeled Nucleotides for Labeling of Isothermal Amplification Products

Authors: Shershov V.E.¹, Kuznetsova V.E.¹, Zasedatelev A.S.¹, Vasiliskov V.A.¹, Shekalova E.Y.¹, Shtylev G.F.¹, Shishkin I.Y.¹, Levashova A.I.¹, Lapa S.A.¹, Chudinov A.V.¹
Affiliations:
1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Issue: Vol 49, No 6 (2023)
Pages: 649-656
Section: ПИСЬМА РЕДАКТОРУ
URL: https://rjsvd.com/0132-3423/article/view/670552
DOI: https://doi.org/10.31857/S0132342323050056
EDN: https://elibrary.ru/BAKVTC
ID: 670552

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Abstract
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Abstract

The synthesis of fluorescently labeled nucleotides containing a zwitterionic indodicarbocyanine dye attached via a trans-alkene spacer at the C5 position of the pyrimidine base was carried out, and their substrate efficiency was tested under conditions of recombinase polymerase amplification (RPA). As a result of RPA, the formation of full-sized target products of the ebpS gene fragment of the causative agent of bacterial pneumonia (Staphylococcus aureus) and a high density of fluorescent label embedding occurred.

Keywords

fluorescently labeled nucleotides, indodicarbocyanine dyes, isothermal amplification, recombinase polymerase reaction

References

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