Isolation of chlorogenic acids and caffeine in the stationary phase of Diaspher-110-C10CN

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Abstract

The transition from the traditional C18(I) stationary phases with nonpolar endcapping to the C10CN(II) phase has been shown to contain the terminal polar group leading to a change in selectivity comparable to an increase in the activity of residual silanol groups in phases I. The effect was found in the separation of isomeric monocopheoylquinic acids. Two variants of gradient modes have been proposed using a column of Diaspher-110-C10CN and aqueous acetonitrile, acidified H3PO4 components of the mobile phase for the separation of chlorogenic acids and caffeine from green coffee extracts from various manufacturers. The proposed chromatographic method has been shown as the method that can be used to determine trigonelin, the retention of which increased markedly when phase I was replaced by phase II. The proposed method is used to differentiate the fruits of two types of coffee – Arabica and robusta. It was found that robusta coffee extracts contain a higher amount of chlorogenic acids and caffeine.

About the authors

V. I. Deyneka

Belgorod State National Research University

Author for correspondence.
Email: deineka@bsu.edu.ru

Institute of Pharmacy, Chemistry and Biology

Russian Federation, Belgorod

E. Yu. Oleynits

Belgorod State National Research University

Email: deineka@bsu.edu.ru

Institute of Pharmacy, Chemistry and Biology

Russian Federation, Belgorod

H. M. Cultid Cabrera

Belgorod State National Research University

Email: deineka@bsu.edu.ru

Institute of Pharmacy, Chemistry and Biology

Russian Federation, Belgorod

L. A. Deyneka

Belgorod State National Research University

Email: deineka@bsu.edu.ru

Institute of Pharmacy, Chemistry and Biology

Russian Federation, Belgorod

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