Multilayer Coatings Based On Citrate-Stabilized Gold Nanoparticles and Polydiallyldimethylammonium Chloride for the Electrophoretic Separation of Carboxylic Acids

D. V. Makeeva; Макеева Д. В.; K. S. Antipova; Антипова К. С.; E. V. Solovyeva; Соловьева Е. В.; V. P. Morgacheva; Моргачева В. П.; E. A. Kolobova; Колобова Е. А.; L. A. Kartsova; Карцова Л. А.

doi:10.31857/S0044450223030088

Multilayer Coatings Based On Citrate-Stabilized Gold Nanoparticles and Polydiallyldimethylammonium Chloride for the Electrophoretic Separation of Carboxylic Acids

Authors: Makeeva D.V.¹, Antipova K.S.¹, Solovyeva E.V.¹, Morgacheva V.P.¹, Kolobova E.A.¹, Kartsova L.A.¹
Affiliations:
1. St. Petersburg State University
Issue: Vol 78, No 3 (2023)
Pages: 241-252
Section: ORIGINAL ARTICLES
Submitted: 31.01.2025
URL: https://rjsvd.com/0044-4502/article/view/650164
DOI: https://doi.org/10.31857/S0044450223030088
EDN: https://elibrary.ru/FUQJKK
ID: 650164

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Abstract

The conditions for the formation of physically adsorbed three-layer coatings of quartz capillary walls in capillary electrophoresis (CE) with successively deposited oppositely charged layers of polydiallyldimethylammonium chloride (PDADMAC) modifiers and citrate-stabilized gold nanoparticles (GNPs) are proposed. It was shown that three-layer PDADMAC–GNP–PDADMAC coatings favorably differ from monolayer coatings with PDADMAC by greater stability in a wide range of pH (2–10). The formed coatings were characterized by scanning electron microscopy, and the presence of a uniform dense layer of nanoparticles on the capillary surface was confirmed. The applicability of the modified capillaries under CE conditions was demonstrated by the separation of a mixture of 16 carboxylic acids. An increase in the separation selectivity achieved with the use of three-layer coatings based on GNPs was explained by the reversible exchange of citrate anions on the GNP surface with negatively charged analytes in the course of electrophoretic analysis.

Keywords

gold nanoparticles, multilayer coatings, capillary electrophoresis, carboxylic acids

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