Vol 100, No 4 (2021)

Title page and table of contents of the Journal

Introduction. The imbalance of macroelements in the soft low-mineralized drinking water of the Primorsky Territory water supply systems can be a risk factor for developing many somatic diseases in the population. 

Aim: hygienic identification of the mineral composition components of drinking water, posing a risk factor for urolithiasis in children, adolescent, and adult populations over a 25-year follow-up period.

Methods include identification of formal statistical relationships between mean annual content of iron, silicon, manganese, calcium, magnesium, sodium, total hardness in drinking water (534 water pipes and 1929 wells), and incidence of urolithiasis in the population (34 administrative areas) by rank correlation analysis, identification cause-effect relations on medical-statistical and hygienic criteria, assessment of the geographical distribution of the attributive risk of urolithiasis at the population level. 

Results. The relation “cause-effect” has been established between the paired ratio of calcium and magnesium in drinking water and incidence of urolithiasis in adults, adolescents, and children. Increased concentrations of silicon, manganese, and iron in drinking water do not affect the studied incidence level. The maximum risk of urolithiasis in the population is mainly located in the North and East of the Primorsky Territory.

Conclusions. An imbalance of calcium and magnesium against a background of magnesium deficiency in tap and well water is the priority risk factor of aquatic origin for urolithiasis in the Primorsky Territory; the features of the geographical distribution of the immediate risk of urolithiasis among the population make it possible to form a scientifically based plan for the sequence of implementation of primary prevention measures for this disease in the region.

Introduction. Today, micromycetes are the most common pathogens found in residential and industrial premises and in the hospital environment. At the same time, mold fungi are the cause not only of allergic diseases but also of mycoses of various localization. Mold fungi are hazardous for patients in hospitals on long-term treatment and exposed to immunosuppression factors. Medical personnel who spent a long time in rooms with high contamination by micromycetes in the air can develop mycogenic sensitization. 

The aim of this work was to show the quantitative and qualitative composition of fungi surrounding the patient and the doctor in multidisciplinary hospitals. 

Materials and methods. With the help of a microbiological aspirator, air samples were taken in the premises of class B of surgical and therapeutic departments, followed by sowing on Saburo agar. The identified fungi were identified using classical methods and MALDI-ToF mass spectrometry using Microflex LT (Bruker). Results. In the course of the study, the assessment of fungal contamination of class B premises in treatment and prophylactic institutions was carried out to assess the qualitative and quantitative composition. Also, comparing the composition and number of micromycetes between the premises of departments of different profiles was carried out. A high level of contamination of micromycetes in medical premises of class B – 100% of cases was revealed in studies of objects of the hospital environment. The structure of the identified fungi was very diverse. In all air samples were isolated fungi genera Aspergillus, Penicillium, Mucor, Alternaria, and Ulcoladium. Spores of these fungi belonging to opportunistic or saprophytes are found in the air everywhere. Still, a significant increase in microbial load due to micromycetes can cause such typical allergic diseases as bronchial asthma, allergic skin dermatoses, and allergies of other locations. 

Conclusion. The study results revealed increased fungal contamination in healthcare facilities that require a more careful approach to compliance with the microclimate parameters.

Introduction. In Russia and abroad, there has been a clear trend towards an increase in cases of sudden death in the workplace from circulatory system diseases over the past decade.

Materials and methods. During the study, the databases of the State Labor Inspectorate in the Republic of Bashkortostan for 2014-2018 were studied, including accident investigation reports, information on a special assessment of working conditions, medical examinations, forensic medical examinations, and other materials.

Results. The leading cause of sudden death in the workplace in more than 90% of cases were circulatory system diseases (CSD). The maximum number of cases of sudden death from CSD was recorded in the age group of 56-60 years. Working condit ions represented the overwhelming majority of the deceased - 82.8%. In 13.7% of cases, they were drivers of transport. The vast majority of deaths occurred in the morning (from 6 to 12) and daytime (from 12 to 18). The working conditions of workers from deceased CSD in most cases (59.8%) corresponded to the permissible class (class 2); under harmful conditions (subclass 3.1-3.3), 24.9% worked. Most often, some form of acute coronary heart disease (CHD) was mentioned as the immediate cause of death (64.0%). Among acute forms of coronary heart disease in medical documents, in more than 50% of cases, code I 24.8 “Other forms of acute coronary heart disease” was established. “Acute myocardial infarction” was verified in 11.1% of patients and was the leading cause of death in men. In the group “Cerebrovascular Diseases,” “Intracerebral hemorrhage” was most often diagnosed.

Conclusion. The obtained results substantiate the need to develop measures to prevent sudden death in the workplace, aimed primarily at preventing the development, progression, and early diagnosis of diseases of the circulatory system.

The purpose of the study was to assess the metabolic status of the body when correcting the diet with a concentrated natural product.

Materials and methods. The diet of athletes was analyzed. The leading group (n = 15) received concentrated food products (CNP) for 20 days: No. 1 - 30.0 g and No. 2 - 20 g each. Blood indices including (total protein, uric acid, urea, creatinine, total cholesterol, high and low-density lipoprotein cholesterol, triglycerides, glucose, amylase) were assessed metabolic status: taken before, after, and in one month of taking CNP 

Results. The diet’s calorie content was 5953.2-6494.4 kcal/day in the main and the comparison group corresponding to this cohort’s physiological need. The proportions of protein and fat exceeded the recommended ones; deficiency of carbohydrates accounted for 8.7%. The inclusion of CNP increased the ratio of slow carbohydrates, vitamins, minerals, and minor food components. In the leading group, the increase in urea and uric acid was less significant in terms of average indices, and the proportion of such people increased. Creatinine was significantly higher than baseline within one month after CNP administration. The increase in the proportion of people with increased total cholesterol is 2.9 times less; the share with a borderline high level decreased by 15.5%, in the comparison group, it increased by 5.6%. The proportion with low-density lipoproteins exceeding the reference value decreased from 61.5% to 30.0% in the comparison group - without dynamics (55.6%) and signs of dyslipidemia increased. In the main group, within the normal range, 76.9% of individuals had an increase in glucose, amylase by 5.7% (p = 0.049).

Conclusion. The diet is not macronutrient balanced. The use of CNP affected the metabolism of proteins, fats, and carbohydrates. It proved a more adequate energy supply for the activities of the persons of the main group. There has been shown the possibility of correcting the body’s metabolic processes with food products that reduces the risk of developing prenosological conditions during physical exertion, particularly fatigue and cardiovascular pathology.

Introduction. An essential task of hygiene is assessing the health status of the population living in areas with adverse environmental and hygienic conditions.

Materials and methods. A study of the health status of 50 children of three age groups was carried out in Dunay city, Primorsky Krai, located near the enterprise “The Far Eastern Center for Radioactive Waste Management, Far East Center “DalRAO.” Health status was assessed using the buccal micronucleus cytome assay (BMCA) to determine the cytogenetic and cytotoxic effects of environmental factors.

Results. The frequency of buccal cells with micronuclei in groups of children from the Dunay city varied within 0.33–0.47‰, which does not exceed the control level of 1.1%, determined within the framework of the international project HMNxl. The level of cytogenetic stress is defined as acceptable (but not low). Given the index of accumulation of cytogenetic damages, it is possible to rank the studied groups according to their cytogenetic status in the direction of deterioration: kindergarten children, older and younger schoolchildren.

Conclusion. The approach proposed in this study made it possible to quantitatively determine the mutagenic and cytotoxic effects of factors on the children’s population of the Dunay city. It can be recommended as an informative method for the population health status assessment during socio-hygienic monitoring, including in the territories of nuclear (legacy) sites.

Introduction. The harmonious development of cognitive processes is a prerequisite for students’ successful mastering of the educational program.

The aim of the study is to give a psychophysiological characteristic of the peculiarities of the formation of cognitive functions in senior students.

Materials and methods. The study of the functional state of the central nervous system (CNS) by the process of variational chronoreflexometry was carried out in 300 senior pupils of the traditional general education school; the assessment of cognitive abilities and intellectual level development was evaluated by using computer tests.

Results. Pupils of the 9^th and 11^th grades were shown to have no significant differences in the CNS functional indices. In contrast, the CNS functional level was 1.6-1.7 times lower than the physiological norm. The nervous reaction’s stability was 1.3 -1.4 times, the level of functionality of the formed functional system was 1.3 times. The decrease in the level of CNS functioning of the studied adolescents was due to the consumption of reserve functional capabilities of the central nervous system and the body as a whole for the formation of cognitive functions, as evidenced by the development of verbal and non-verbal thinking in students from the 9^th to the 11^th grade, high speed of thinking processes, voluntary attention and its stability. It was proved that the development of thinking contributed to the formation of voluntary attention, an increase in its stability, and the degree of concentration, confirmed by the established correlation of moderate strength between the accuracy index and the level of formation of various types of thinking (r = 0,4).

Conclusion. The established features of students’ intellectual development during a three-year study can be taken into account when organizing the educational process in traditional educational institutions to increase the level of cognitive development and academic performance of students.

Introduction. Electronic devices (ED) are widespread among adolescents, filling their daily lives. The trend towards digitalization of all aspects of life is a global trend with the inevitable involvement of young people. The lack of existing preventive measures necessitates scientific research and intensification of work to promote a healthy lifestyle.

Materials and methods. In 2019, an online survey was conducted of 200 schoolchildren in grades 9-11, 498 students in 1-6 courses, and 251 parents. In April 2020, an online survey was conducted of 1587 students of 1-6 classes and 500 parents. Measurements of physical factors were carried out at the Dolgoprudnenskaya gymnasium and the N.I. Pirogov Russian National Research Medical University. One thousand sixty-six schoolchildren and students were examined by an ophthalmologist with the help of the device “ARMISm”.

Results. Negative tendencies in the state of the organ of vision, observed by students, are primarily associated with increased use of stationary and mobile ED in all age groups. Students use various ED in places with insufficient artificial illumination. In educational institutions, such places are corridors, halls, recreation, where students use their electronic devices. There are currently no separate requirements for artificial lighting by using information and communication technologies outside of classrooms or work premises. During the period of distance learning, schoolchildren and students have been established to use one more ED in comparison with the period of traditional education. The long-term effect of using mobile ED is still unknown.

Conclusions. As a preventive hygienic measure for the protection of vision, it is recommended to use ED only in places with a good level of illumination, including artificial, with limited duration of continuous use of ED, with gymnastics for the eyes during breaks.

Introduction. The article presents the findings of a study of the adaptive responses of working people depending on their professional activity nature.

The purpose of the study is to evaluate the adaptive response of working people exposed to harmful and dangerous factors. 

Material and methods. The research involved 225 people, including 157 cases exposed to different hazards and dangers: Chernobyl nuclear power plant disaster relief workers (their average age was 59.2 ± 2.2 years (M ± m)); servicemen working with rocket fuels (their average age was 30.6 ± 1.3 years (M ± m)); and service members participated in combat operations (their average age was 32.0 ± 0.8 years (M ± m)). A set of standard methods was used to assess their cardiorespiratory system’s functional state, biological age, activity levels in different organs, and adaptive abilities.

Results. The study has made it possible to describe distribution in terms of ANS response, biological age, adaptive potential, and their correlation. The relief workers showed a medium inverse correlation between adaptive potential and ANS response type (r = -0.308; р = 0.153) and a significant medium positive correlation between adaptive potential and biological age (r = 0.690; р < 0.001). The servicemen working with rocket fuels showed a high medium positive correlation (r = 0.726; р = 0.001) and a significant medium positive correlation (r = 0.658; р < 0.004) between adaptive potential and ANS response type and biological age, respectively. The servicemen who had participated in combat operations showed weak inverse correlation (r = -0.139; р = 0.155) and medium positive correlation (r = 0.683; р = 0.005) in the first and second case, respectively.

Conclusion. The biological age, individual aging rate, ANS response, and potential adaptive performance can be used as criteria for assessing health risks for working people exposed to different hazards and dangers and determining groups of people who need case follow-up.

Introduction. Given the significant prevalence of lead in the environment, research in this area has significant social and economic importance. Lead compounds are characterized by high toxicity and increased ability to cumulate in ecosystems, humans, and animals. Lead enters the human body with food, drinking water, atmospheric air, and smoking. Lead causes pathological changes in the nervous system, blood-forming organs, kidneys, etc.

Materials and methods. The experiments were carried out on four groups of white female rats, each included seven animals, weighing 150-200 g. The first group of animals was a control. The second group consumed dechlorinated water from the city water supply, followed by lead acetate. The animals from the third and fourth groups drank the same water with sodium stearate and potassium stearate content in a dose of 1/250 LD₅₀. After the 40th-day of the use of these waters, the animals were orally administered lead acetate at a dose of 7 mg/kg. The levels of lipid peroxidation biomarkers were studied by studying the content of diene conjugates (DC) and malondialdehyde (MDA) in blood serum, liver, and kidney homogenates.

Results. The administration of 1/2 acetate LD₅₀ to lead in experimental rats drinking water with stearates was accompanied by a significant increase in the DCs concentration and (MDA) in animals. Higher concentrations of LPO products were observed in the group of animals that consumed water from potassium stearate.

Conclusions. 1. With the oral administration of lead acetate against the background of drinking water containing stearates at a dose of 1/250 LD₅₀, an increase in lipid peroxidation indices was noted compared with the control group. 2. Higher concentrations of LPO products were observed in the group of animals consuming water from potassium stearate.