The various dose-dependent effect of selenium oxide and copper oxide nanoparticles in vitro and application of the hormesis paradigm

Introduction. In vitro studies on a culture of cardiomyocytes have shown that dose-response relationships could be monotonic for some effects and non-monotonic for others. In this work, we wanted to demonstrate that these features of the dose-response relationship are a general pattern.

Materials and methods. In vitro experiments were conducted on the culture of human fibroblast-like cells FLECH-104. The cytotoxicity of spherical nanoparticles of selenium oxide (SeO-NP) and copper oxide (CuO-NP) was studied with an average diameter of 51 ± 14 nm and 21 ± 4 nm, respectively.

Results. SeO-NP and CuO-NP were cytotoxic for human fibroblast-like cells, as judged by a decrease in ATP-dependent luminescence. In this case, the cytotoxicity of CuO-NP was somewhat more substantial than the SeO-NP one. Our experiment revealed doses that cause both cell hypertrophy and a decrease in the size of cells and nuclei.

Discussion. We observed both monotonic and different variants of the non-monotonic dose-response relationship. For the latter, it was possible to construct adequate mathematical expressions based on the generalized hormesis paradigm that we had considered earlier concerning the CdS-NP and PbS-NP cytotoxicity for cardiomyocytes.

Conclusion. The general rule is the variability of the dose-response dependence types manifested in different cytotoxic effects of nanoparticles.

Contribution:

Panov V.G. — statistical and mathematical processing, writing a text.

Minigalieva I.A. — collection of literature data, writing a text, editing.

Bushueva T.V. — writing a text.

Artemenko E.P. — collection of literature data, collection and processing of material.

Ryabova Iu.V. — collection of literature data, collection and processing of material.

Sutunkova M.P. — the concept and design of the study.

Gurvich V.B. — the concept and design of the study.

Privalova L.I. — the concept and design of the study, editing.

Katsnelson B.A. — the concept and design of the study, editing.

All authors are responsible for the integrity of all parts of the manuscript and approval of the manuscript final version.

Conflict of interests. The authors declare no conflict of interests.

Acknowledgments. The study had no sponsorship.

The conclusion of the committee on biomedical ethics: the Local Ethics Committee of the Yekaterinburg Medical Research Center for Prophylaxis and Health Protection in Industrial Workers, Russian Agency for Consumer Rights Protection, protocol number 2 of 20.04.21.

Received: November 10, 2021 / Accepted: November, 2021 / Published: December 30, 2021