Transcriptional Activity of CCA 1 in Northern Population Arabidopsis thaliana Plants under Altered Light Conditions

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

The dynamics of the transcriptional activity of one of the key genes of the circadian network, CCA1, was analyzed under conditions of a natural light photoperiod of a long day (16L : 8D) and under an inverted light regime (8D : 16L) in A. thaliana plants of the northern natural population (Karelia). It has been shown that under conditions of an inverted shift in the light regime, there is a sharp increase in the expression of this gene with a phase shift in the circadian rhythm by 2 hours. The level of CCA1 transcriptional activity was almost two times higher compared to the natural light conditions. At the same time, the endogenous rhythm of the gene was preserved, but with a smaller amplitude. With age, 30-day-old plants grown under inverted conditions experienced a loss of endogenous CCA1 circadian rhythm. The results obtained allow us to conclude that the circadian rhythms of A. thaliana, northern natural populations, probably play an important role in adaptation to changing light conditions, and that one of the key clock genes, CCA1, plays a significant role in this process.

Full Text

Restricted Access

About the authors

M. V. Zaretskaya

Institute of Biology of Karelian Research Centre Russian Academy of Sciences

Author for correspondence.
Email: genmg@mail.ru
Russian Federation, Petrozavodsk, 185910

О. M. Fedorenko

Institute of Biology of Karelian Research Centre Russian Academy of Sciences

Email: fedorenko_om@mail.ru
Russian Federation, Petrozavodsk, 185910

References

  1. Zhu Z., Quint M., Anwer M.U. Arabidopsis EARLY FLOWERING 3 controls temperature responsiveness of the circadian clock independently of the evening complex // J. Exp. Bot. 2022. V. 73. № 3. P. 1049–1061. https://doi.org/10.1093/jxb/erab473
  2. Venkat A., Muneer S. Role of circadian rhythms in major plant metabolic and signaling pathways // Front. in Plant Sci. 2022. V. 13. https://doi.org/10.3389/fpls.2022.836244
  3. Ueda H.R. Systems biology flowering in the plant clock field // Mol. Syst. Biol. 2006. V. 2(60). https://doi.org/10.1038/msb4100105
  4. Yamashino T., Ito S., Niwa Y. et al. Involvement of Arabidopsis clock-associated pseudo-response regulators in diurnal oscillations of gene expression in the presence of environmental time cues // Plant Cell Physiol. 2008. V. 49(12). P. 1839–1850. https://doi.org/10.1093/pcp/pcn165
  5. Ronald J., Davis S.J. Making the clock tick: The transcriptional landscape of the plant circadian clock // F1000Res. 2017. V. 6(951). https://doi.org/10.12688/f1000research.11319.1
  6. Flis A., Fernández A.P., Zielinski T. et al. Defining the robust behaviour of the plant clock gene circuit with absolute RNA timeseries and open infrastructure // Open Biol. 2015. V. 5(10). https://doi.org/10.1098/rsob.150042
  7. Linde A.M., Eklund D.M., Kubota A. et al. Early evolution of the land plant circadian clock // New Phytol. 2017. V. 216. P. 576–590. https://doi.org/10.1111/nph.14487
  8. Suárez-López P., Wheatley K., Robson F. et al. G. CONSTANS mediates between the circadian clock and the control of flowering in Arabidopsis // Nature. 2001. V. 410(6832). P. 1116–1120. https://doi.org/10.1038/35074138
  9. Sugiyama H., Natsui Y., Hara M. et al. Late flowering phenotype under ultra-short photoperiod (USP) in Arabidopsis thaliana // Plant Biotechnology. 2014. V. 31(1). P. 29–34. https://doi.org/10.5511/plantbiotechnology.13.1104a
  10. Rees H., Joynson R., Brown J.K.M. et al. Naturally occurring circadian rhythm variation associated with clock gene loci in Swedish Arabidopsis accessions // Plant Cell Eniron. 2021. V. 44. P. 807–820. https://doi.org/10.1111/pce.13941
  11. Anwer M.U., Davis A., Davis S.J., Quint M. Photoperiod sensing of the circadian clock is controlled by EARLY FLOWERING 3 and GIGANTEA // Plant J. 2020. V. 101(6). P. 1397–1410. https://doi.org/ 10.1111/tpj.14604
  12. Sawa M., Kay S.A. GIGANTEA directly activates FLOWERING LOCUS T in Arabidopsis thaliana // Proc. Natl Acad. Sci. USA. 2011. V. 108. P. 11698–11703. https://doi.org/10.1073/pnas.1106771108
  13. Salathia N., Davis S.J., Lynn J.R. et al. FLOWERING LOCUS C-dependent and -independent regulation of the circadian clock by the autonomous and vernalization pathways // BMS Plant Biol. 2006. V. 6. № 10. https://doi.org/10.1186/1471-2229-6-10
  14. Nitschke S., Cortleven A., Iven T., Feussner I. et al. Circadian stress regimes affect the circadian clock and cause jasmonic acid-dependent cell death in ctokinin-deficient Arabidopsis plants // Plant Cell. 2016. V. 28(7). P. 1616–1639. https://doi.org/10.1105/tpc.16.00016
  15. Иванов В.И., Касьяненко А.Г., Санина А.В. и др. Краткая характеристика A. thaliana и некоторые сведения о его культивировании, технике скрещиваний и учете изменчивости // Генетика. 1966. Т. 8. № 1. С. 115–120.
  16. Livak K.J., Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2–∆∆Ct method // Methods. 2001. V. 25. P. 402–408. https://doi: 10.1006/meth.2001.1262
  17. Квитко К.В., Мюллер А. Новый объект для генетических исследований – Arabidopsis thaliana (L.) Heynh. // Исследования по генетике. Т. 1. Л.: Изд-во ЛГУ. 1961. С. 79.
  18. Fujiwara S., Oda A., Yoshida R. et al. Circadian clock proteins LHY and CCA1 regulate SVP protein accumulation to control flowering in Arabidopsis // Plant Cell. 2008. V. 20(11). P. 2960–2971. https://doi: 10.1105/tpc.108.061531
  19. Millar J., Carrington J.T., Tee W. et al. Changing planetary rotation rescues the biological clock mutant lhy cca1 of Arabidopsis thaliana // bioRxiv. 2015. https://doi.org/10.1101/034629

Supplementary files

Supplementary Files
Action
1. JATS XML
Download
2. Fig. 1. Graphic representation of the conditions for growing plants under long daylight hours: (a) – seeds were sown under natural photoperiod (16L : 8D); light was on from 6 a.m. to 10 p.m.; 20-day-old plants (Ш20) were analyzed. (b) – plants from group “a” were grown for 10 days under inverted light conditions (8D : 16L); light was on from 5 p.m. to 9 a.m.; 30-day-old plants (Ш30) were analyzed. (c, d) – seeds were sown under inverted light conditions (8D : 16L); 20-day-old (C20) and 30-day-old (C30) plants were analyzed.

Download (55KB)
Indexing metadata
3. Fig. 2. Dynamics of CCA1 transcriptional activity in 20-day-old A. thaliana plants of the Shuiskaya (Ш20) and Ler populations grown under natural photoperiod conditions of 16L:8D (light was turned on at 6 am). Note. Here and in Figs. 3 and 4: X-axis – time of day in hours; Y-axis – relative level of CCA1 transcripts.

Download (67KB)
Indexing metadata
4. Fig. 3. Dynamics of CCA1 transcriptional activity in A. thaliana plants of the Shuiskaya population grown under inverted light conditions of 8D:16L (light was turned on at 5 p.m.). a – 30-day-old plants grew under inverted light conditions (Ш30) only for the last 10 days; b and c – 20-day-old (С20) and 30-day-old (С30) plants constantly grown under inverted light conditions.

Download (162KB)
Indexing metadata
5. Fig. 4. Dynamics of CCA1 transcriptional activity in A. thaliana plants of the Shuyskaya population grown under inverted light conditions of 8D : 16L (light continues from 5 p.m. to 9 a.m.). a – plants grew under natural light photoperiod conditions (16L : 8D) (Ш20) (data are given from Fig. 1 for comparison); b – 30-day-old plants grew under inverted light conditions only for the last 10 days (Ш30); c and d – 20-day-old (С20) and 30-day-old (С30) plants constantly grown under inverted light conditions.

Download (198KB)
Indexing metadata

Copyright (c) 2024 Russian Academy of Sciences