Transferability of SSR Markers Developed on Gosseberry to Red and Black Currants

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Abstract

Eleven microsatellite markers previously developed based on gooseberry sequences were tested on red and black currants. As a result, all microsatellite loci were amplified on representatives of black currant, but on representatives of red currant there was no amplification in three loci (RucANS, RucDFR2-1, RucDFR1-3). Polymorphic loci have been identified for both black and red currants. In the MTT-7 locus in the studied black currant genotypes, amplification of 3 fragments has been observed. Probably this locus is duplicated in black currant genome. At the same time, in red currant, amplification typical for monolocus microsatellite has been observed in the MTT-7 locus. The RucHLH-1 and RucUFGT loci were tested on a hybrid red currant family (Belaya Potapenko × 1426-21-80). By genetic mapping, the localization of the RucHLH-1 locus was established on linkage group 4 in the red currant genome, and the localization of RucUFGT was presumably on linkage group 1. The set of microsatellite loci for the currant genus is currently limited. This work shows that some of the SSR markers developed on gooseberries are amplified and reveal polymorphism in currants and can be used to study both black and red currants.

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A. V. Pikunova

All-Russian Research Institute of Fruit Crop Breeding, VNIISPK

Author for correspondence.
Email: pikuanna84@mail.ru
Russian Federation, Zhilina, Orel district, 302530

A. A. Pavlenko

All-Russian Research Institute of Fruit Crop Breeding, VNIISPK

Email: pikuanna84@mail.ru
Russian Federation, Zhilina, Orel district, 302530

M. A. Dolzhikova

All-Russian Research Institute of Fruit Crop Breeding, VNIISPK

Email: pikuanna84@mail.ru
Russian Federation, Zhilina, Orel district, 302530

O. D. Golyaeva

All-Russian Research Institute of Fruit Crop Breeding, VNIISPK

Email: pikuanna84@mail.ru
Russian Federation, Zhilina, Orel district, 302530

S. D. Knyazev

All-Russian Research Institute of Fruit Crop Breeding, VNIISPK

Email: pikuanna84@mail.ru
Russian Federation, Zhilina, Orel district, 302530

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2. Fig. 1. Genetic map of linkage group 4 of red currant and localization of the RucHLH-1 locus on it (indicated by arrow).

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