Interrelation between Composition of Natural Water and a State of Lipid Peroxidation Processes in Biological Objects

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Abstract

The interrelation between the composition of components of five samples of natural water and state indices for the lipid peroxidation regulatory system in a model system based on the natural phospholipids was explored and involvement of natural phospholipids in the formation of the toxicity of the natural water was also studied. It is shown that the presence of N- and P-containing compounds in natural water samples leads to inhibition of the processes relevant to lecithin autooxidation and luminescence intensity of luminous bacteria, has a significant effect on the spontaneous aggregation of lecithin, while an increase in the content of hydrophobic compounds results in a higher negative value of the ξ potential of its particles. High sensitivity of the lipid peroxidation regulatory system to the presence of components even at low concentrations in the natural water makes it a promising tool to test the effect of natural water on biological objects. Mathematical processing of UV spectra of the natural water samples with the Gauss method can be used as an express test for the analysis of its hydrochemical composition. The effects of natural water components on the state of membranes of biological objects and intracellular processes are confirmed by means of biotesting methods.

About the authors

L. N Shishkina

N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Email: shishkina@sky.chph.ras.ru
Moscow, Russia

A. S Dubovik

N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Moscow, Russia

V. O Shvydkyi

N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Moscow, Russia

M. V Kozlov

N.M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences

Moscow, Russia

E. V Shtamm

N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences

Moscow, Russia

A. G Georgiadi

Institute of Geography, Russian Academy of Sciences

Moscow, Russia

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