The place of laser fluorescence spectroscopy, doppler flowmetry and ultrasound in the diagnosis and assessment of treatment efficacy for plaque scleroderma
- Authors: Khynku E.F.1, Raznitsyna I.A.1, Molochkova Y.V.1, Rogatkin D.A.1, Selesneva E.V.1, Glazkov A.A.1, Molochkov A.V.1
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Affiliations:
- Moscow Regional Research and Clinical Institute
- Issue: Vol 25, No 4 (2022)
- Pages: 289-302
- Section: DERMATOLOGY
- URL: https://rjsvd.com/1560-9588/article/view/109060
- DOI: https://doi.org/10.17816/dv109060
- ID: 109060
Cite item
Abstract
According to modern ideas, a reasonable choice of an effective method of treating plaque scleroderma is based on the diagnosis of the pathological process prevailing in the tissues (inflammation-sclerosis). Therefore, an urgent problem of a personalized approach to dermatosis therapy is the possibility of an objective assessment of the prevailing process using non-invasive diagnostic methods. The article presents a clinical case of widespread plaque scleroderma in a 66-year-old patient, demonstrating the possibility of using laser fluorescence spectroscopy and laser Doppler flowmetry to determine the degree of activity of the focus and determine the leading pathological process.
We selected three pathological skin foci localized in the abdomen and characterizing three clinical stages of the disease (inflammation, induration, sclerosis). The analysis of fluorescence and laser Doppler flowmetry data showed that in areas clinically defined as inflammation, there is an increase in the average values of the indices of tissue content of porphyrins, lipofuscin and microcirculation index compared with intact skin, while the intensity of collagen fluorescence does not differ significantly. In the induration zone, along with an increase in the fluorescence indices of lipofuscin and porphyrins, there is an increase in the average values of collagen fluorescence indices at effective registration waves. The data obtained by us indicate an active inflammatory process in these foci and the process of fibrosis in the induration zone. In the sclerosis zone, there is an increase in the average values of collagen fluorescence indices compared with intact skin, and the fluorescence of optical markers of inflammation (lipofuscin and porphyrins) do not differ significantly in comparison with the control intact skin. When analyzing the fluorescence spectra and laser Doppler flowmetry data after treatment, we found that in the zones of induration and inflammation, the average values of the fluorescence indices of porphyrins, lipofuscin, collagen and microcirculation index are reduced relative to the initial values (before treatment), but remain higher in comparison with intact skin. The data obtained may indicate that active inflammation in these foci persists at the time of the study. In the study of the focus of sclerosis, the obtained autofluorescence and microcirculation data in dynamics do not differ significantly from the initial values. The data for laser Doppler flowmetry and laser fluorescence spectroscopy are consistent with ultrasound examination of the skin.
In our study, the potential possibility of using laser fluorescence spectroscopy and laser Doppler flowmetry methods to establish the degree of activity of the focus, determine the leading pathological process, as well as to evaluate the effectiveness of therapy was demonstrated for the first time.
Full Text
About the authors
Evgeniy F. Khynku
Moscow Regional Research and Clinical Institute
Author for correspondence.
Email: Khynky_ev@mail.ru
ORCID iD: 0000-0003-4368-8469
SPIN-code: 2530-1273
Russian Federation, Moscow
Irina A. Raznitsyna
Moscow Regional Research and Clinical Institute
Email: RaznitsynaIA@yandex.ru
ORCID iD: 0000-0003-4145-6947
SPIN-code: 9092-4490
Junior Research Associate
Russian Federation, MoscowYulia V. Molochkova
Moscow Regional Research and Clinical Institute
Email: yulia-molochkova@yandex.ru
ORCID iD: 0000-0001-9021-6494
SPIN-code: 2051-0250
MD, Dr. Sci. (Med.), Assistant Professor
Russian Federation, MoscowDmitry A. Rogatkin
Moscow Regional Research and Clinical Institute
Email: rogatkin@medphyslab.com
ORCID iD: 0000-0002-7755-308X
SPIN-code: 9130-8111
http://www.medphyslab.ru
MD, Dr. Sci. (Med.), Assistant Professor
Russian Federation, MoscowElena V. Selesneva
Moscow Regional Research and Clinical Institute
Email: Selezneva-elena@mail.ru
ORCID iD: 0000-0002-6181-9031
SPIN-code: 9748-6169
MD, Cand. Sci. (Med.), Assistant Lecturer
Russian Federation, MoscowAlexey A. Glazkov
Moscow Regional Research and Clinical Institute
Email: staaglz@gmail.com
ORCID iD: 0000-0001-6122-0638
SPIN-code: 3250-1882
MD, Cand. Sci. (Med.)
Russian Federation, MoscowAnton V. Molochkov
Moscow Regional Research and Clinical Institute
Email: antmd@yandex.ru
ORCID iD: 0000-0002-6456-998X
SPIN-code: 1883-4452
MD, Dr. Sci. (Med.), Professor
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