Russian Journal of Skin and Venereal DiseasesRussian Journal of Skin and Venereal DiseasesРоссийский журнал кожных и венерических болезней1560-95882412-9097Eco-Vector3697910.17816/dv36979ArticlesСтатьиResearch ArticleT-cell receptor repertory in some immunassociated dermatosesРепертуар Т-клеточных рецепторов при некоторых иммунозависимых дерматозахParamonovA. AПарамоновАлексей Александрович

Department of Cutaneous and Sexually-Transmitted Diseases, Therapeutic Faculty

Кафедра кожных и венерических болезней лечебного факультета; аспирант

paramonov_aleksey@mail.ruKayumovaL. NКаюмоваЛяиля Наилевна

Department of Cutaneous and Sexually-Transmitted Diseases, Therapeutic Faculty

Кафедра кожных и венерических болезней лечебного факультета; аспирант

avestohka2005@inbox.ruBruskinS. AБрускинСергей Александрович

кандидат биол. наук, доцент

brouskin@vigg.ruKocherginNicolay G.КочергинНиколай Георгиевич

MD, PhD, DSc, prof.

доктор мед. наук, профессор

nkocha@yandex.ruOlisovaO. YuОлисоваОльга Юрьевна

Department of Cutaneous and Sexually-Transmitted Diseases, Therapeutic Faculty

Кафедра кожных и венерических болезней лечебного факультета; доктор мед. наук, профессор

olisovaolga@mail.ruOstretsovaM. NОстрецоваМария Николаевна

Department of Cutaneous and Sexually-Transmitted Diseases, Therapeutic Faculty

Кафедра кожных и венерических болезней лечебного факультета; аспирант

mostretsova@gmail.comI.M. Setchenov First Moscow State Medical UniversityГБОУ ВПО Первый МГМУ им. И.М. Сеченова Минздрава РоссииN.I. Vavilov Institute of GeneticsФГБУН Институт общей генетики им. Н.И. Вавилова РАН15082015184VOL 18, NO4 (2015)ТОМ 18, №4 (2015)344121072020Copyright ©; 2015, Eco-VectorCopyright ©; 2015, ООО "Эко-Вектор"2015Eco-VectorООО "Эко-Вектор"https://rjsvd.com/1560-9588/article/view/36979

The repertory of T-cell receptors in some immunassociated cutaneous disease is presented. We studied the T-cell receptor variety in involved and intact skin and in the blood of patients with psoriasis and atopic dermatitis and the time course of these receptors during immunosuppressive therapy by massive sequencing methods, in order to detect the probable specific antigen as a potential target for developing new methods for effective therapy of these diseases.

Представлены результаты исследований по изучению многообразия (репертуара) Т-клеточных рецепторов при некоторых иммунозависимых кожных заболеваниях. В нашем исследовании изучен репертуар Т-клеточных рецепторов в пораженной и непораженной коже, а также в крови при псориазе и атопическом дерматите, а также установлен характер изменений данного репертуара у больных в ходе иммуносупрессивной терапии при помощи методов массивного секвенирования с целью выявления возможного специфического антигена в качестве потенциальной мишени для разработки новых методов эффективной терапии данных заболеваний.

T-cell receptorsT-cellssclerodermalupus erythemarosisvitiligoacneT-cell lymphomapsoriasisatopic dermatitisТ-клеточныерецепторыТ-клеткипсориазатопический дерматитсклеродермиякрасная волчанкавитилигоакнеТ-клеточная лимфомаNomura T., Kabashima K., Miyachi Y. The panoply of αβT cells in the skin. J. Dermatol. Sci. 2014; 76(1): 3-9. doi: 10.1016/j.jdermsci.2014.07.010.Murphy K., Travers P., Walport M. Janeway’s Immunobiology. New York: Garland Science; 2011.Alberts B., Johnson A., Lewis J., Raff M., Roberts K., Walter P. Molecular Biology of the Cell. New York: Garland Science; 2002.Adams J.J., Narayanan S., Liu B., Birnbaum M.E., Kruse A.C., Bowerman N.A., et al. T cell receptor signaling is limited by docking geometry to peptide-major histocompatibility complex. Immunity. 2011; 35(5): 681-93. doi: 10.1016/j.immuni.2011.09.013.Voorhees J.J. Pathophysiology of psoriasis. Annu. Rev. Med. 1977; 28: 467-73.Mueller W., Herrmann B. Cyclosporin A for psoriasis. N. Engl. J. Med. 1979; 301(10): 555.Mrowietz U. Cyclosporine as maintenance therapy in patients with severe psoriasis. J. Am. Acad. Dermatol. 2013; 69(2): 308-9. doi: 10.1016/j.jaad.2012.09.005.Swimberghe S., Ghislain P.D., Daci E., Allewaert K., Denhaerynck K., Hermans C., et al. Clinical, quality of life, patient adherence, and safety outcomes of short-course (12 weeks) treatment with cyclosporine in patients with severe psoriasis (the practice study). Ann. Dermatol. 2013; 25(1): 28-35. doi: 10.5021/ad.2013.25.1.28.Nicolas J.F., Chamchick N., Thivolet J., Wijdenes J., Morel P., Revillard J.P. CD4 antibody treatment of severe psoriasis. Lancet. 1991; 338(8762): 321.Prinz J., Braun-Falco O., Meurer M., Daddona P., Reiter C., Rieber P., Riethmüller G. Chimaeric CD4 monoclonal antibody in treatment of generalised pustular psoriasis. Lancet. 1991; 338(8762): 320-21.Abrams J.R., Kelley S.L., Hayes E., Kikuchi T., Brown M.J., Kang S., et al. Blockade of T lymphocyte costimulation with cytotoxic T lymphocyte-associated antigen 4-immunoglobulin (CTLA4Ig) reverses the cellular pathology of psoriatic plaques, including the activation of keratinocytes, dendritic cells, and endothelial cells. J. Exp. Med. 2000; 192(5): 681-94.Wrone-Smith T., Nickoloff B.J. Dermal injection of immunocytes induces psoriasis. J. Clin. Invest. 1996; 98(8): 1878-87. doi:10.1172/JCI118989.Lewis H.M., Baker B.S., Bokth S., Powles A.V., Garioch J.J., Valdimarsson H., Fry L. Restricted T-cell receptor V beta gene usage in the skin of patients with guttate and chronic plaque psoriasis. Br. J. Dermatol. 1993; 129(5): 514-20.Leung D.Y., Travers J.B., Giorno R., Norris D.A., Skinner R., Aelion J., et al. Evidence for a streptococcal superantigen-driven process in acute guttate psoriasis. J. Clin. Invest. 1995; 96(5): 2106-12.Telfer N.R., Chalmers R.J., Whale K., Colman G. The role of streptococcal infection in the initiation of guttate psoriasis. Arch. Dermatol. 1992; 128(1): 39-42.Gudjonsson J.E., Thorarinsson A.M., Sigurgeirsson B., Kristinsson K.G., Valdimarsson H. Streptococcal throat infections and exacerbation of chronic plaque psoriasis: a prospective study. Br. J. Dermatol. 2003; 149(3): 530-4.Cai Y., Fleming C., Yan J. New insights of T cells in the pathogenesis of psoriasis. Cell. Mol. Immunol. 2012; 9(4): 302-9. doi: 10.1038/cmi.2012.15.Prinz J.C. Disease mimicry - a pathogenetic concept for T cell-mediated autoimmune disorders triggered by molecular mimicry? Autoimmun. Rev. 2004; 3(1): 10-5.Prinz J.C. Psoriasis vulgaris-a sterile antibacterial skin reaction mediated by cross-reactive T cells? An immunological view of the pathophysiology of psoriasis. Clin. Exp. Dermatol. 2001; 26(4): 326-32.Prinz J.C., Vollmer S., Boehncke W.H., Menssen A., Laisney I., Trommler P. Selection of conserved TCR VDJ rearrangements in chronic psoriatic plaques indicates a common antigen in psoriasis vulgaris. Eur. J. Immunol. 1999; 29(10): 3360-8.Vollmer S., Menssen A., Prinz J.C. Dominant lesional T cell receptor rearrangements persist in relapsing psoriasis but are absent from nonlesional skin: evidence for a stable antigen-specific pathogenic T cell response in psoriasis vulgaris. J. Invest. Dermatol. 2001; 117(5): 1296-301.Ye P., Rodriguez F.H., Kanaly S., Stocking K.L., Schurr J., Schwarzenberger P., et al. Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense. J. Exp. Med. 2001; 194(4): 519-27.Huang W., Na L., Fidel P.L., Schwarzenberger P. Requirement of interleukin-17A for systemic anti-Candida albicans host defense in mice. J. Infect. Dis. 2004; 190(3): 624-31.Elloso M.M., Gomez-Angelats M., Fourie A.M. Targeting the Th17 pathway in psoriasis. J. Leukoc. Biol. 2012; 92(6): 1187-97. doi:10.1189/jlb.0212101.Antiga E., Volpi W., Cardilicchia E., Maggi L., Filì L., Manuelli C., et al. Etanercept downregulates the Th17 pathway and decreases the IL-17+/IL-10+ cell ratio in patients with psoriasis vulgaris. J. Clin. Immunol. 2012; 32(6): 1221-32. doi: 10.1007/s10875-012-9716-x.Quaglino P., Bergallo M., Ponti R., Barberio E., Cicchelli S., et al. Th1, Th2, Th17 and regulatory T cell pattern in psoriatic patients: modulation of cytokines and gene targets induced by etanercept treatment and correlation with clinical response. Dermatology. 2011; 223(1): 57-67. doi: 10.1159/000330330.Benham H., Norris P., Goodall J., Wechalekar M.D., FitzGerald O., Szentpetery A., et al. Th17 and Th22 cells in psoriatic arthritis and psoriasis. Arth. Res. Ther. 2013; 15(5): R136. doi: 10.1186/ar4317.Nishimoto S., Kotani H., Tsuruta S., Shimizu N., Ito M., Shichita T., et al. Th17 cells carrying TCR recognizing epidermal autoantigen induce psoriasis-like skin inflammation. J. Immunol. 2013; 191(6): 3065-72. doi: 10.4049/jimmunol.1300348.O’Brien R.L., Born W.K. Dermal γδ T cells - What have we learned? Cell. Immunol. 2015. pii: S0008-8749(15)00012-X. doi: 10.1016/j.cellimm.2015.01.011.Bata-Csorgo Z., Hammerberg C., Voorhees J.J., Cooper K.D. Kinetics and regulation of human keratinocyte stem cell growth in short-term primary ex vivo culture. Cooperative growth factors from psoriatic lesional T lymphocytes stimulate proliferation among psoriatic uninvolved, but not normal, stem keratinocytes. J. Clin. Invest. 1995; 95(1): 317-27.Kelsen J., Dige A., Christensen M., D’Amore F., Iversen L. Frequency and clonality of peripheral γδ T cells in psoriasis patients receiving anti-tumour necrosis factor-αtherapy. Clin. Exp. Immunol. 2014; 177(1): 142-8. doi: 10.1111/cei.12331.Kono F., Honda T., Aini W., Manabe T., Haga H., Tsuruyama T. Interferon-γ/CCR5 expression in invariant natural killer T cells and CCL5 expression in capillary veins of dermal papillae correlate with development of psoriasis vulgaris. Br. J. Dermatol. 2014; 170(5): 1048-55. doi: 10.1111/bjd.12812.Cairo C., Arabito E., Landi F., Casati A., Brunetti E., Mancino G., Galli E. Analysis of circulating γδ+ T cells in children affected by IgE-associated and non-IgE-associated allergic atopic eczema/dermatitis syndrome. Clin. Exp. Immunol. 2005; 141(1): 116-21. doi:10.1111/j.1365-2249.2005.02813.x.Goldsby R.A., Kindt T.J., Osborne B.A., Kuby J. Immunology. New York: W.H. Freeman and Company; 2003.Schauer U., Dippel E., Gieler U., Brӓuer J., Jung T., Heymanns J., Rieger C.H. T cell receptor gamma delta bearing cells are decreased in the peripheral blood of patients with atopic diseases. Clin. Exp. Immunol. 1991; 86(3): 440-3.Katsuta M., Takigawa Y., Kimishima M., Inaoka M., Takahashi R., Shiohara T. NK cells and γδ+T cells are phenotypically and functionally defective due to preferential apoptosis in patients with atopic dermatitis. J. Immunol. 2006; 176(12): 7736-44. doi:10.4049/jimmunol.176.12.7736Matsuoka A., Kato T., Soma Y., Takahama H., Nakamura M., Matsuoka H., Mizoguchi M. Analysis of T cell receptor (TCR) BV-gene clonotypes in NC/Nga mice developing dermatitis resembling human atopic dermatitis. J. Dermatol. Sci. 2005; 38(1): 17-24.Imura K., Yoshioka T., Hikita I., Hirasawa T., Sakata T., Matsutani T., et al. Association of T-cell receptor Vbeta haplotypes with dry skin in DS-Nh mice. Clin. Exp. Dermatol. 2009; 34(1): 61-7. doi:10.1111/j.1365-2230.2008.02921.x.Yoshioka T., Imura K., Hikita I., Hirasawa T., Sakata T., Matsutani T., et al. Impact of T-cell receptor Vbeta haplotypes on the development of dermatitis in DS-Nh mice: synergistic production of interleukin13 caused by staphylococcal enterotoxin C and peptide glycans from Staphylococcus aureus. Immunology. 2007; 121(1): 51-61.Woodward A.L., Spergel J.M., Alenius H., Mizoguchi E., Bhan A.K., Castigli E., et al. An obligate role for T-cell receptor alphabeta+ T cells but not T-cell receptor gammadelta+ T cells, B cells, or CD40/CD40L interactions in a mouse model of atopic dermatitis. J. Allergy Clin. Immunol. 2001; 107(2): 359-66.Wedderbum L.R., O’Hehir R.E., Hewitt C.R., Lamb J.R., Owen M.J. In vivo clonal dominance and limited T-cell receptor usage in human CD4+ T-cell recognition of house dust mite allergens. Proc. Natl. Acad. Sci. USA. 1993; 90(17): 8214-8.Neuber K., Löliger C., Köhler I., Ring J. Preferential expression of T-cell receptor V beta-chains in atopic eczema. Acta. Derm. Venereol. 1996; 76(3): 214-8.Yudate T., Yamada H., Tezuka T. Role of staphylococcal enterotoxins in pathogenesis of atopic dermatitis: growth and expression of T cell receptor V beta of peripheral blood mononuclear cells stimulated by enterotoxins A and B. J. Dermatol. Sci. 1996; 13(1): 63-70.Torres M.J., Gonzalez F.J., Corzo J.L., Giron M.D., Carvajal M.J., Garcia V., et al. Circulating CLA+ lymphocytes from children with atopic dermatitis contain an increased percentage of cells bearing staphylococcal-related T-cell receptor variable segments. Clin. Exp. Allergy. 1998; 28(10): 1264-72.Ha S.J., Lee H.J., Byun D.G., Kim J.W. Expression of T cell receptor V beta chain in lesional skin of atopic dermatitis. Acta. Derm. Venereol. 1998; 78(6): 424-7.Johansson C., Jeddi-Tehrani M., Grunewald J., Tengvall Linder M., Bengtsson A., Halldén G., Scheynius A. Peripheral blood T-cell receptor beta-chain V-repertoire in atopic dermatitis patients after in vitro exposure to Pityrosporum orbiculare extract. Scand. J. Immunol. 1999; 49(3): 293-301.Riccieri V., Parisi G., Spadaro A., Scrivo R., Barone F., Moretti T., et al. Reduced circulating natural killer T cells and gamma/delta T cells in patients with systemic sclerosis. J. Rheumatol. 2005; 32(2): 283-6.Giacomelli R., Cipriani P., Fulminis A., Nelson J.L., Matucci-Cerinic M. Gamma/delta T cells in placenta and skin: their different functions may support the paradigm of microchimerism in systemic sclerosis. Clin. Exp. Rheumatol. 2004; 22(3, Suppl. 33): S28-30.Sakkas L.I., Xu B., Artlett C.M., Lu S., Jimenez S.A., Platsoucas C.D. Oligoclonal T cell expansion in the skin of patients with systemic sclerosis. J. Immunol. 2002; 168(7): 3649-59.Mak A., Kow N.Y. The pathology of T cells in systemic lupus erythematosus. J. Immunol. Res. 2014; 2014: 419029. doi: 10.1155/2014/419029.Barrera-Vargas A., Gómez-Martín D., Alcocer-Varela J. T cell receptor-associated protein tyrosine kinases: the dynamics of tolerance regulation by phosphorylation and its role in systemic lupus erythematosus. Hum. Immunol. 2014; 75(9): 945-52. doi: 10.1016/j.humimm.2014.08.207.McDonald G., Deepak S., Miguel L., Hall C.J., Isenberg D.A., Magee A.I., et al. Normalizing glycosphingolipids restores function in CD4+ T cells from lupus patients. J. Clin. Invest. 2014; 124(2): 712-24. doi: 10.1172/JCI69571.Li X., Kang N., Zhang X., Dong X., Wei W., Cui L., et al. Generation of human regulatory gammadelta T cells by TCRγδ stimulation in the presence of TGF-beta and their involvement in the pathogenesis of systemic lupus erythematosus. J. Immunol. 2011; 186(12): 6693-700. doi: 10.4049/jimmunol.1002776.Wang L., Kang N., Zhou J., Guo Y., Zhang X., Cui L., et al. Downregulation of CD94/NKG2A inhibitory receptor on decreased γδ T cells in patients with systemic lupus erythematosus. Scand. J. Immunol. 2012; 76(1): 62-9. doi: 10.1111/j.1365-3083.2012.02705.x.Lu Z., Su D., Wang D., Li X., Feng X., Sun L. Elevated apoptosis and impaired proliferation contribute to downregulated peripheral γδ T cells in patients with systemic lupus erythematosus. Clin. Dev. Immunol. 2013; 2013: 405395. doi: 10.1155/2013/405395.Luo W., Ma L., Yao X.S., Zou H.Y., Wen Q., Ruan G.P., Wang X.N. Complementarity-determining region 3 analysis of T cell receptor beta chain variable region in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. Nan Fang Yi Ke Da Xue Xue Bao. 2006; 26(8): 1128-31.Luo W., Ma L., Wen Q., Wang N., Zhou M.Q., Wang X.N. Analysis of the interindividual conservation of T cell receptor alpha- and betachain variable regions gene in the peripheral blood of patients with systemic lupus erythematosus. Clin. Exp. Immunol. 2008; 154(3): 316-24. doi: 10.1111/j.1365-2249.2008.03770.x.Wang H.X., Chu S., Li J., Lai W.N., Wang H.X., Wu X.J., et al. Increased IL-17 and IL-21 producing TCRαβ+CD4-CD8- T cells in Chinese systemic lupus erythematosus patients. Lupus. 2014; 23(7): 643-54.Al-Harthi L., Marchetti G., Steffens C.M., Poulin J., Sékaly R., Landay A. Detection of T cell receptor circles (TRECs) as biomarkers for de novo T cell synthesis using a quantitative polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA). J. Immunol. Methods. 2000; 237(1-2): 187-97.Kurosaka D., Yasuda J., Ikeshima-Kataoka H., Ozawa Y., Yoshida K., Yasuda C. Decreased numbers of signal-joint T cell receptor excision circle-containing CD4+ and CD8+ cells in systemic lupus erythematosus patients. Mod. Rheumatol. 2007; 17(4): 296-300.Gregg R.K., Nichols L., Chen Y., Lu B., Engelhard V.H. Mechanisms of spatial and temporal development of autoimmune vitiligo in tyrosinase-specific TCR transgenic mice. J. Immunol. 2010; 184(4): 1909-17. doi: 10.4049/jimmunol.0902778.Holland D.B., Cunliffe W.J., Morrison J.F.J. Oligoclonality in T-cell receptor Vβ gene usage in acne lesions. J. Invest. Dermatol. 1995; 105(3): 482.Jappe U., Ingham E., Henwood J., Holland K.T. Propionibacterium acnes and inflammation in acne; P. acnes has T-cell mitogenic activity. Br. J. Dermatol. 2002; 146(2): 202-9.Юсупова Л.А., Гараева З.Ш., Юнусова Е.И., Мавлютова Г.И. Первичная эпидермотропная Т-клеточная лимфома кожи. Лечащий врач. 2013; 6: 54-9.Cherny S., Mraz S., Su L., Harvell J., Kohler S. Heteroduplex analysis of T-cell receptor gamma gene rearrangement as an adjuvant diagnostic tool in skin biopsies for erythroderma. J. Cutan. Pathol. 2001; 28(7): 351-5.Yamanaka K., Fuhlbrigge R.C., Mizutani H., Kupper T.S. Restoration of peripheral blood T cell repertoire complexity during remission in advanced cutaneous T cell lymphoma. Arch. Dermatol. Res. 2010; 302(6): 453-9. doi:10.1007/s00403-009-1023-x.