Russian Journal of Skin and Venereal DiseasesRussian Journal of Skin and Venereal Diseases1560-95882412-9097Eco-Vector3691410.17816/dv36914Research ArticleGenodiagnosis of HPV beta DNA association with seborrheic keratosis in immunosuppressed and immunocompetent patientsKornevaL. Vlvkorneva@mail.ruSnarskayaElena S.MD, PhD, DSc, prof.snarskaya-dok@mail.ruMolochkovV. A-PolyanskayaA. A-M.F. Vladimirsky Moscow Regional Research and Clinical InstituteI.M. Setchenov First Moscow State Medical UniversityPremium Esthetics Clinic150420151824721072020Copyright © 2015, Eco-Vector2015Numerous data indicate the involvement of human papilloma virus (HPV), genus beta, in the development of epithelial tumors of the skin. We studied the association of seborrheic keratosis (SK) with HPV by analysis of HPV beta DNA, including that in immunosuppressed patients. Twenty recipients of renal transplants and 20 immunocompetent patients with SK were examined. Control group consisted of 49 volunteers without epithelial tumors of the skin. Genodiagnosis of HPV beta DNA in biopsy specimens was carried out by the real time polymerase chain reaction with hybridization fluorescent detection. Amplification and detection were carried out on a Rotor-Gene 3000 device (Corbett Research, Australia). The results indicated high levels of HPV DNA in transplant recipients - in foci of SK and in visually intact skin (in 85 and 55% cases, respectively), which was higher than the incidence of the virus in donor skin (47%). Virus charge in SKfoci was significantly higher than in the control group.genus beta human papilloma virusgenodiagnosis of HPVcutaneous epithelial tumorsseborrheic keratosisrenal transplant recipientsimmunosuppressionвирус папилломы человека рода betaгенодиагностика ДНК вируса папилломы человекаэпителиальные опухоли кожисеборейный кератозреципиенты почечного трансплантатаиммуносупрессия[IARC. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans. Human Papillomaviruses. Lyon; 2012. vol. 90.][Accardi R., Gheit T. Cutaneous HPV and skin cancer. Presse Med. 2014; 43(12P2): e435-43.][Кладова А.Ю., Куевда Д.А., Молочков В.А., Шипулина О.Ю., Киселев В.И., Хлебникова А.Н., Козлова Е.С. Встречаемость кожных типов вирусов папилломы человека в опухолях кожи. Альманах клинической медицины. МОНИКИ. 2006; 9: 44-50.][Quint K.D., Genders R.E., de Koning M.N., Borgogna C., Gariglio M., Bouwes Bavinck J.N., Doorbar J., Feltkamp M.C. Human beta-papillomavirus infection and keratinocyte carcinomas. J. Pathol. 2015; 235(2): 342-54.][Borgogna C., Lanfredini S., Peretti A., De Andrea M., Zavattaro E., Colombo E. et al. Improved detection reveals active beta-papillomavirus infection in skin lesions from kidney transplant recipients. Mod Pathol. 2014; 27(8): 1101-15.][Farzan S.F., Waterboer T., Gui J., Nelson H.H., Li Z., Michael K.M., et al. Cutaneous alpha, beta and gamma human papillomaviruses in relation to squamous cell carcinoma of the skin: a population-based study. Int. J. Cancer. 2013; 133(7): 1713-20.][Zhao Y.K., Lin Y.X., Luo R.Y., Huang X.Y., Liu M.Z., Xia M., Jin H. Human papillomavirus (HPV) infection in seborrheic keratosis. Am. J. Dermatopathol. 1989; 11(3): 209-12.][Li Y.H., Chen G., Dong X.P., Chen H.D. Detection of epidermodysplasia verruciformis-associated human papillomavirus DNA in non-genital seborrhoeic keratosis. Br. J. Dermatol. 2004; 151(5): 1060-5.][Meibodi N.T., Nahidi Y., Meshkat Z., Esmaili H., Gharib M., Gholoobi A. N evidence of human papillomaviruses in non-genital seborrheic keratosis. Indian J. Dermatol. 2013; 58(4): 326. doi: 10.4103/0019-5154.113949.]