Russian Journal of Skin and Venereal DiseasesRussian Journal of Skin and Venereal DiseasesРоссийский журнал кожных и венерических болезней1560-95882412-9097Eco-Vector10632710.17816/dv106327DERMATO-ONCOLOGYДЕРМАТООНКОЛОГИЯResearch ArticleMicroRNA as a diagnostic marker in cutaneous T-cell lymphomasМикроРНК как диагностический маркер при Т-клеточных лимфомах кожиhttps://orcid.org/0000-0003-2482-17542500-7989OlisovaOlga Yu.ОлисоваОльга Юрьевна
Russian Federation

MD, Dr. Sci. (Med.), Professor

д.м.н., профессор

olisovaolga@mail.ruhttps://orcid.org/0000-0002-3408-61005130-8270DemkinVladimir V.ДемкинВладимир Витальевич
Russian Federation

MD, Cand. Sci. (Med.)

к.м.н.

vdemkin@img.ras.ruhttps://orcid.org/0000-0002-0827-40522683-1517ChernovaNatalia G.ЧерноваНаталья Геннадьевна
Russian Federation

MD, Cand. Sci. (Med.)

к.м.н.

ngchernova@mail.ruhttps://orcid.org/0000-0002-3907-21896770-5019AmshinskayaJessika R.АмшинскаяДжессика Рафаэлевна
Russian Federation

Graduate Student

аспирант

dr.jessika@yandex.ruhttps://orcid.org/0000-0002-5559-6003KazakovAndrey A.КазаковАндрей Аркадьевич
Russian Federation
andrey20079@mail.ruI.M. Sechenov First Moscow State Medical University (Sechenov University)Первый Московский государственный медицинский университет имени И.М. Сеченова (Сеченовский Университет)Institute of Molecular Genetics of the Russian Academy of SciencesИнститут молекулярной генетикиCity Clinical Hospital № 40Городская клиническая больница № 40030820222515161604202218052022Copyright ©; 2022, Olisova O.Y., Demkin V.V., Chernova N.G., Amshinskaya J.R., Kazakov A.A.Copyright ©; 2022, Олисова О.Ю., Демкин В.В., Чернова Н.Г., Амшинская Д.Р., Казаков А.А.2022Olisova O.Y., Demkin V.V., Chernova N.G., Amshinskaya J.R., Kazakov A.A.Олисова О.Ю., Демкин В.В., Чернова Н.Г., Амшинская Д.Р., Казаков А.А.https://creativecommons.org/licenses/by-nc-nd/4.0/https://rjsvd.com/1560-9588/article/view/106327

BACKGROUND: In recent years, thanks to the development of methods of molecular genetic analysis, microRNA has become one of the promising markers for the diagnosis of many human diseases.

AIMS: to study microRNA as a new method for the diagnosis of fungal mycosis.

MATERIALS AND METHODS: The study included 30 patients with histologically confirmed diagnosis of T-cell lymphomas of the skin, 25 were diagnosed with fungal mycosis, 5 ― Cesari syndrome. The control group consisted of 10 patients with benign lymphoproliferative dermatoses. The patients underwent the determination of microRNA 223, 16, 326, 663, 423, 711 in blood plasma. MicroRNA was also detected in plasma in patients with T-cell lymphomas of the skin at early and late stages.

RESULTS: Statistically significant difference of 223, 16, 326, 711 microRNAs in blood plasma was revealed in patients with fungal mycosis, compared with patients with benign lymphoproliferative dermatoses. Statistically significant difference of 663 microRNA in blood plasma was revealed in patients with T-cell lymphomas of the skin at early and late stages. A statistically significant difference of 223, 711 microRNAs in blood plasma was revealed in patients with fungal mycosis at an early stage compared with patients with benign lymphoproliferative dermatoses.

CONCLUSION: The determination of microRNA 223, 16, 326, 711 in blood plasma can be used for early diagnosis of T-cell lymphomas of the skin.

Обоснование. В последние годы благодаря развитию методов молекулярно-генетического анализа одним из перспективных маркеров для диагностики многих заболеваний человека становится микроРНК.

Цель ― изучение микроРНК в плазме крови для ранней диагностики грибовидного микоза.

Материал и методы. В исследование были включены 30 пациентов с гистологически подтверждённым диагнозом Т-клеточной лимфомы кожи, у 25 был диагностирован грибовидный микоз, у 5 ― синдром Сезари. Группу контроля составили 10 пациентов с доброкачественными лимфопролиферативными дерматозами. Пациентам проводилось определение микроРНК 223, 16, 326, 663, 423, 711 в плазме крови. Проведено также определение микроРНК в плазме у пациентов с Т-клеточными лимфомами кожи на ранних и поздних стадиях.

Результаты. Выявлена статистически значимая разница микроРНК 223, 16, 326, 711 в плазме крови у пациентов с грибовидным микозом в сравнении с пациентами с доброкачественными лимфопролиферативными дерматозами, микроРНК 663 у пациентов с Т-клеточными лимфомами кожи на ранней и поздней стадиях, а также микроРНК 223, 711 у пациентов с грибовидным микозом на ранней стадии в сравнении с пациентами с доброкачественными лимфопролиферативными дерматозами.

Заключение. Определение микроРНК 223, 16, 326, 711 в плазме крови может быть использовано для ранней диагностики Т-клеточной лимфомы кожи.

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