R- and S-Pyruvate-Containing Polysaccharide from the Cell Wall of Rathayibacter sp. VKM Ac-2927

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The structures of two cell wall glycopolymers from the strain Rathayibacter sp. VKM Ac-2927 (family Microbacteriaceae, class Actinomycetes), isolated from linden leaves affected by leaf miner, were established using chemical and NMR spectroscopy methods. The first polymer, rhamnomannan, is a heterogeneous chain polymer composed of regularly alternating α-D-rhamnose and α-D-mannose residues linked by (1→3)- and (1→2)-glycosidic bonds and contains a minor amount of lateral β-D-xylopyranose residues (the structure is presented in the text). The second polymer, acetalized with R- and S-pyruvic acid, consists of branched tetrasaccharide units, →3)-α-D-Galp4,6-(R-Pyr)-(1→3)-β-D-Glcp-(1→6)-[β-D-Galp3,4-(S-Pyr)-(1→4)]-α-D-Manp-(1→. The structure of this polysaccharide is new for representatives of the genus Rathayibacter and prokaryotes in general. The results of the present study indicate the structural diversity of microbial glycopolymers and are consistent with previously obtained data on the specificity of their composition for species of the genus Rathayibacter.

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作者简介

M. Kokoulin

Elyakov Pacific Institute of Bioorganic Chemistry of the Far Eastern Branch of the Russian Academy of Sciences

Email: potekhina56@mail.ru
俄罗斯联邦, Vladivostok

N. Potekhina

Lomonosov Moscow State University

Email: potekhina56@mail.ru
俄罗斯联邦, Moscow

E. Tulskaya

Lomonosov Moscow State University

Email: potekhina56@mail.ru
俄罗斯联邦, Moscow

Yu. Ospennikov

Skryabin Institute of Biochemistry and Physiology of Microorganisms

Email: potekhina56@mail.ru
俄罗斯联邦, Pushchino

L. Evtushenko

Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, All-Russian Collection of Microorganisms (VKM)

编辑信件的主要联系方式.
Email: potekhina56@mail.ru
俄罗斯联邦, Pushchino, Moscow Region

参考

  1. Evtushenko, L. I., and Dorofeeva, L. V. (2012) Genus XXII. Rathayibacter Zgurskaya, Evtushenko, Akimov and Kalakoutskii 1993, 147VP in Bergey’s manual of systematic bacteriology (Goodfellow, M., Kämpfer, P., Busse, H-J., Trujillo, M. E., Suzuki, K.-I., Ludwig, W., Whitman, W. B., eds) 2nd Edn, vol. 5. Springer, New York, Dordrecht, Heidelberg, London, pp. 953-964.
  2. Potekhina, N. V., Tul’skaya, E. M., Ospennikov, Y. V., and Evtushenko, L. I. (2023) Cell wall glycopolymers as chemotaxonomic characteristics of the genus and species Rathayibacter, Microbiology, 92, S2-S6, https://doi.org/10.1134/S0026261723603822.
  3. Shashkov, A. S., Potekhina, N. V., Tul’skaya, E. M, Dmitrenok, A. S., Senchenkova, S. N., Torgov, V. I., Dorofeeva, L. V., and Evtushenko, L. I. (2024) New lactate- and pyruvate-containing polysaccharide and rhamnomannan with xylose residues from the cell wall of Rathayibacter oskolensis VKM Ac-2121T, Carbohydr. Res., 540, 109145, https://doi.org/10.1016/j.carres.2024.109145.
  4. Шашков А. С., Потехина Н. В, Тульская Е. М., Дмитренок А. С., Сенченкова С. Н., Дорофеева Л. В., Евтушенко Л. И. (2024) Новые рамнозосодержащие гликополимеры клеточной стенки Rathayibacter festucae ВКМ Ac-1390T, Биохимия, 89, 2081-2091, https://doi.org/10.31857/S0320972524120051.
  5. Tarlachkov, S. V., Ospennikov, Y. V., Demidov, A. V., Starodumova, I. P., Dorofeeva, L. V., Prisyazhnaya, N. V., et al. (2022) Draft genome sequences of 9 actinobacteria from the family Microbacteriaceae associated with insect- and nematode-damaged plants, Microbiol. Res. Announc., 11, e00487-22, https://doi.org/10.1128/mra.00487-22.
  6. Potekhina, N. V., Streshinskaya, G. M., Tul’skaya, E. M., and Shashkov, A. S. (2011) In Methods in Microbiology (Rainey, F. A., and Oren, A., eds), Vol. 38, Academic Press/Elsevier, Amsterdam, pp. 131-164, https://doi.org/10.1016/B978-0-12-387730-7.00020-6.
  7. Dmitrenok, A. S., Streshinskaya, G. M., Tul’skaya, E. M., Potekhina, N. V., Senchenkova, S. N., Shashkov, A. S., Bilan, M. I., Starodumova, I. P., Bueva, O. V., and Evtushenko, L. I. (2017) Pyruvylated cell wall glycopolymers of Promicromonospora citrea VKM Ac-665T and Promicromonospora sp. VKM Ac-1028, Carbohydr. Res., 449, 34-142, https://doi.org/10.1016/j.carres.2017.07.008.
  8. Leontein, K., Lindberg, B., and Lonngren, J. (1978) Assignment of absolute configuration of sugars by g.l.c. of their acetylated glycosides formed from chiral alcohols, Carbohydr. Res., 62, 359-362, https://doi.org/10.1016/S0008-6215(00)80882-4.
  9. Shashkov, A. S., Lipkind, G. M., Knirel, Y. A., and Kochetkov, N. K. (1988) Stereometrical factors determining the effects of glycosylation on the 13C chemical shifts in carbohydrates, Magn. Reson. Chem., 26, 735-747, https://doi.org/10.1002/mrc.1260260904.
  10. Garegg, P.J., Jansson, P.-E., Lindberg, B., Lindh, F., and Lönngren, J. (1980) Configuration of the acetal carbon atom of pyruvic acid acetals in some bacterial polysaccharides, Carbohydr. Res., 79, 127-132, https://doi.org/10.1016/S0008-6215(00)83666-6.
  11. Chun, J., Oren, A., Ventosa, A., Christensen, H., Arahal, D. R., da Costa M. S., Rooney, A. P., Yi, H., Xu, X. W., De Meyer, S., and Trujillo, M. E. (2018) Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes, Int. J. Syst. Evol. Microbiol., 68, 461-466, https://doi.org/10.1099/ijsem.0.002516.

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2. Fig. 1. 13C NMR spectra of glycopolymers from the cell wall of Rathayibacter sp. VKM Ac-2927: initial preparation (a); neutral fraction (b); acidic fraction (c)

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3. Fig. 2. 1H,13C HSQC NMR spectrum of the neutral fraction of the cell wall glycopolymers of Rathayibacter sp. VKM Ac-2927. The corresponding parts of the 1H and 13C NMR spectra are shown above and to the left of the two-dimensional spectrum, respectively. Arabic numerals refer to the numbers of carbon atoms in residues labelled with capital Latin letters according to Table 1

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4. Fig. 3. Parts of two-dimensional 1H,1H ROESY (a) and 1H,13C HMBC (b) spectra of the neutral fraction of the cell wall glycopolymers of Rathayibacter sp. VKM Ac-2927. The corresponding parts of the 1H and 13C NMR spectra are shown above and to the left of the two-dimensional spectrum, respectively. Arabic numerals refer to the numbers of carbon atoms in residues labelled with capital Latin letters according to Table 1

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5. Formula 1. Structure of the repeating units of a neutral polysaccharide

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6. Fig. 4. 1H,13C HSQC NMR spectrum of the acidic fraction of glycopolymers from the cell wall of Rathayibacter sp. VKM Ac-2927. The corresponding parts of the 1H and 13C NMR spectra are shown above and to the left of the two-dimensional spectrum, respectively. Arabic numerals refer to the numbers of carbon atoms in residues labelled with capital Latin letters according to Table 1

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7. Fig. 5. Parts of the two-dimensional 1H,1H ROESY spectrum of the acidic fraction of glycopolymers from the cell wall of Rathayibacter sp. VKM Ac-2927. The corresponding parts of the 1H and 13C NMR spectra are shown above and to the left of the two-dimensional spectrum, respectively. Arabic numerals refer to the numbers of carbon atoms in residues labelled with capital Latin letters according to Table 1

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8. Formula 2. Structure of the repeating link of an acidic polysaccharide

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