Аnalyzing buckwheat genotypes obtained on selective media with zinc in vitro using ISSR markers

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The paper evaluates four ISSR-markers (М1, М2, М7, and М11) for their effectiveness in research on the genetic distances of regenerated Fagopyrum esculentum plants, which were obtained in vitro on selective media with high doses of ZnSO4×7 H2O (808–1313 mg/L). The level of polymorphism was determined to be high in the combined sample (74.4%). The obtained speciemens might be used in the breeding of new varieties with economically important traits.

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Sobre autores

S. Borovaya

Chaikа Federal Scientific Center of Agricultural Biotechnology of the Far East

Autor responsável pela correspondência
Email: borovayasveta@mail.ru
Rússia, Primorsky Krai, Timiriasevsky, 692539

A. Klykov

Chaikа Federal Scientific Center of Agricultural Biotechnology of the Far East

Email: borovayasveta@mail.ru
Rússia, Primorsky Krai, Timiriasevsky, 692539

N. Boginskaya

Chaikа Federal Scientific Center of Agricultural Biotechnology of the Far East

Email: borovayasveta@mail.ru
Rússia, Primorsky Krai, Timiriasevsky, 692539

Bibliografia

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  5. Meloni M., Perini D., Filigheddu R., Binelli G. Genetic variation in five Mediterranean populations of Juniperus phoenicea as revealed by inter-simple sequence repeat (ISSR) markers // Ann. Bot. 2006. V. 97. № 2. P. 299–304. https://doi.org/10.1093/aob/mcj024
  6. Alhasnawi A.N., Kadhimi А.А., Isahak А. et al. Application of inter simple sequence repeat (ISSR) for detecting genetic analysis in rice (Oryza sativa L.) // J. Pure Appl. Microbiology. 2015. V. 9. № 2. P. 1091–11016.
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  10. Sabreena N. М., Mahajan R., Hashim M.J. et al. Deciphering allelic variability and population structure in buckwheat: An analogy between the efficiency of ISSR and SSR markers // Saudi J. Biol. Sci. 2021. V. 28. Р. 6050–6056. https://doi.org/10.1016/j.sjbs.2021.07.061
  11. Shukla A., Srivastava N., Suneja P. et al. Genetic diversity analysis in Buckwheat germplasm for nutritional traits // Indian J. Experim. Biology. 2018. V. 56. № 11. Р. 827–837.

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1. JATS XML
2. Fig. 1. UPGMA is a dendrogram based on the values of genetic distances for buckwheat regenerants. 1 is a group of variants characterized by the smallest DN compared to the original form; 2 is a group of variants characterized by the largest DN compared to the original form.

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